Effect of membrane fluidity on tyrosine kinase activity of reconstituted epidermal growth factor receptor

被引：30

作者：

Ge, GX ^{[1
]}

Wu, J ^{[1
]}

Lin, QS ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2001年 / 282卷 / 02期

关键词：

epidermal growth factor (EGF); EGF receptor; tyrosine kinase; reconstitution; liposome; membrane fluidity; alamethicin;

D O I：

10.1006/bbrc.2001.4600

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Epidermal growth factor receptor (EGFR) was functionally reconstituted into liposome membrane. Triton X-100 was removed by Bio-beads SM-2, More than 80% of the reconstituted EGFR possessed right-side-out orientation with the EGF binding side facing the medium. The tyrosine kinase assay of the EG;FR was carried out in the presence of the antibiotic alamethicin, The reconstituted EGFR tyrosine kinase was well activated by EGF. The influence of lipid composition on tyrosine kinase activity was investigated, Introduction of cholesterol into the dioleoylphophatidylcholine (DOPC) liposome membrane resulted in the decrease of tyrosine kinase activity. The tyrosine kinase activity of EGFR in distearylphosphatidylcholine liposome was much lower than that of EGFR-DOPC proteoliposome, Results indicated the importance of membrane fluidity on the apparent tyrosine kinase activity of reconstituted EGFR. (C) 2001 Academic Press.

引用

页码：511 / 514

页数：4

共 20 条

[1] INHIBITION OF TYROSINE KINASE-ACTIVITY OF THE EPIDERMAL GROWTH-FACTOR (EGF) RECEPTOR BY A TRUNCATED RECEPTOR FORM THAT BINDS TO EGF - ROLE FOR INTERRECEPTOR INTERACTION IN KINASE REGULATION [J].