Phenotypic and genotypic discrepancy of Streptococcus pneumoniae strains isolated from Asian countries

被引：12

作者：

Ko, KS

Oh, WS

Peck, KR

Lee, JH

Lee, NY

Song, JH ^{[1
]}

机构：

[1] ARFID, Seoul, South Korea

[2] Sungkyunkwan Univ, Sch Med, Div Infect Dis, Samsung Med Ctr, Seoul, South Korea

[3] Sungkyunkwan Univ, Sch Med, Dept Lab Med, Samsung Med Ctr, Seoul, South Korea

来源：

FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY | 2005年 / 45卷 / 01期

关键词：

Streptococcus pneumoniae; MLST; optochin; bile solubility; atypical pneumococci;

D O I：

10.1016/j.femsim.2005.01.012

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Non-typeable isolates of Streptococcus pneumoniae collected from Asian countries were characterized by optochin susceptibility test, bile solubility test, multilocus sequence typing of housekeeping genes, amplification of virulence-related genes, 16S rDNA-Rsa1 digestion. and 16S rDNA sequencing. Six of 54 non-typeable pneumococcal isolates showed divergence of gene sequences of recP and xpt from typical pneumococcal strains. Of these six atypical pneumococcal strains, two showed different results in optochin susceptibility or bile solubility test from typical pneumococcal strains. All six isolates showed high sequence dissimilarities of multilocus sequence typing, 16S rDNA sequences, and lytA sequences from typical S. pneumoniae strains. Data from this study suggest that classic tests such as optochin susceptibility and bile solubility tests may lead to incorrect identification of S. pneumoniae. These atypical strains may belong to different bacterial species from S. pneumoniae. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：63 / 70

页数：8

共 28 条

[1]

[Anonymous], 1992, CLIN MICROBIOLOGY PR

[2] Accuracy of phenotypic and genotypic testing for identification of Streptococcus pneumoniae and description of Streptococcus pseudopneumoniae sp nov. [J].