Identification of peptide sequences that induce the transport of phage across the gastrointestinal mucosal barrier

被引:55
作者
Duerr, DM
White, SJ [1 ]
Schluesener, HJ
机构
[1] Univ Bristol, Bristol Royal Infirm, Bristol Heart Inst, Bristol BS2 8HW, Avon, England
[2] Univ Tubingen, Brain Res Inst, D-72076 Tubingen, Germany
关键词
macromolecular transport; peptide; in vivo phage display; intestinal mucosa;
D O I
10.1016/j.jviromet.2003.11.012
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
To investigate whether specific peptide sequences could induce virion transport across the intestinal barrier, we used phage display to both identify signalling peptides capable of inducing trans-intestinal transport and also provide a suitable model of virion translocation. We utilised simple, single-round high input in vivo biopanning protocol using a 7-mer random amino acid phage display library. Phage were applied by gavage and translocation across the intestinal barrier assessed by phage recovery from the spleen 2 h later. Following isolation, a number of phage were sequenced and several homologies with HIV gp120 were identified. Inummocytochemical analysis of phage translocation across the intestinal barrier by a phage bearing the peptide YPRLLTP demonstrated that phage were actively transported along specific channels. It is concluded that utilisation of in vivo phage display (IVPD) has provided evidence for a specific peptide-guided transport of undegraded cargo across the intestinal barrier, modelled by M13 phage. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码:177 / 180
页数:4
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