A new series of mycobacterial expression vectors for the development of live recombinant vaccines

被引:8
作者
Baulard, A
Kremer, L
Supply, P
Vidaud, D
Bidart, JM
Bellet, D
Locht, C
机构
[1] INST PASTEUR,LAB MICROBIOL GENET & MOL,INSERM,CJF9109,F-59019 LILLE,FRANCE
[2] INST PASTEUR,CTR IMMUNOL & BIOL PARASITAIRE,INSERM,U167,LILLE,FRANCE
[3] CNRS,LAB IMMUNOL TUMEURS,URA1484,F-75006 PARIS,FRANCE
关键词
Mycobacterium bovis BCG; shuttle vectors; mercury resistance; 85A antigen promoter; human choriogonadotropin; bladder cancer;
D O I
10.1016/0378-1119(96)00239-9
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Recombinant BCG (bacillus Calmette-Guerin) is a promising candidate as a live vaccine delivery system. Thus far, however, only autoreplicative plasmids carrying the heterologous genes to be expressed in BCG, together with antibiotic-resistance genes, have been successfully used. This could potentially lead to the spreading of antibiotic resistance among other bacteria, and might therefore be unsafe for the environment. In this study, we present a series of three Escherichia coli-Mycobacteria shuttle vectors which enable expression and secretion of antigens without the use of antibiotic-resistance markers. All these plasmids confer mercury resistance to the host bacteria as the only selectable marker and contain a unique restriction site to allow for single-step in-frame cloning of open reading frames downstream from the Mycobacterium tuberculosis 85A antigen promoter and export signal. The system was used to express the free beta-subunit of human chorionic gonadotropin (hCG beta), a potential target of an immunotherapeutic vaccine.
引用
收藏
页码:149 / 154
页数:6
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