Genome transplantation in bacteria: Changing one species to another

被引：282

作者：

Lartigue, Carole ^{[1
]}

Glass, John I. ^{[1
]}

Alperovich, Nina ^{[1
]}

Pieper, Rembert ^{[1
]}

Parmar, Prashanth P. ^{[1
]}

Hutchison, Clyde A., III ^{[1
]}

Smith, Hamilton O. ^{[1
]}

Venter, J. Craig ^{[1
]}

机构：

[1] J Craig Venter Inst, Rockville, MD 20850 USA

来源：

SCIENCE | 2007年 / 317卷 / 5838期

关键词：

D O I：

10.1126/science.1144622

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

As a step toward propagation of synthetic genomes, we completely replaced the genome of a bacterial cell with one from another species by transplanting a whole genome as naked DNA. Intact genomic DNA from Mycoplasma mycoides large colony (LC), virtually free of protein, was transplanted into Mycoplasma capricolum cells by polyethylene glycol-mediated transformation. Cells selected for tetracycline resistance, carried by the M. mycoides LC chromosome, contain the complete donor genome and are free of detectable recipient genomic sequences. These cells that result from genome transplantation are phenotypically identical to the M. mycoides LC donor strain as judged by several criteria.

引用

页码：632 / 638

页数：7

共 24 条

[1] Incorporation of the whole chromosomal DNA in protoplast lysates into competent cells of Bacillus subtilis [J].