An enzyme-linked immunosorbent assay (ELISA) for the measurement of activated factor XII (Hageman factor) in human plasma

被引：45

作者：

Ford, RP ^{[1
]}

Esnouf, MP ^{[1
]}

Burgess, AI ^{[1
]}

Sarphie, A ^{[1
]}

机构：

[1] RADCLIFFE INFIRM,NUFFIELD DEPT CLIN BIOCHEM,OXFORD OX2 6HE,ENGLAND

来源：

JOURNAL OF IMMUNOASSAY | 1996年 / 17卷 / 02期

关键词：

human activated factor XII; monoclonal antibody; enzyme-linked immunosorbent assay;

D O I：

10.1080/01971529608005783

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A direct enzyme-linked immunosorbent assay (ELISA) employing 2/215 mouse monoclonal hybridoma antibody is described. The assay is capable of detecting activated factor XII in human plasma and can be used to assess early detection of the intrinsic blood coagulation pathway. No cross reactivity with human factor XII zymogen has been found. The assay was used to assess activation of factor XII in patients with renal failure, pregnancy and diabetes compared to a control group.

引用

页码：119 / 131

页数：13

共 9 条

[1] MEASUREMENTS OF THE TRUE AFFINITY CONSTANT IN SOLUTION OF ANTIGEN-ANTIBODY COMPLEXES BY ENZYME-LINKED IMMUNOSORBENT-ASSAY [J].

FRIGUET, B ;

CHAFFOTTE, AF ;

DJAVADIOHANIANCE, L ;

GOLDBERG, ME .

JOURNAL OF IMMUNOLOGICAL METHODS, 1985, 77 (02) :305-319

[2]

FUJIKAWA K, 1983, J BIOL CHEM, V258, P924

[3]

FUJIKAWA K, 1981, METHOD ENZYMOL, V80, P198

[4]

HALBMAYER WM, 1994, THROMB HAEMOSTASIS, V71, P68

[5] ENZYME-LABELING OF ANTIBODIES AND THEIR FRAGMENTS FOR ENZYME-IMMUNOASSAY AND IMMUNOHISTOCHEMICAL STAINING [J].

ISHIKAWA, E ;

IMAGAWA, M ;

HASHIDA, S ;

YOSHITAKE, S ;

HAMAGUCHI, Y ;

UENO, T .

JOURNAL OF IMMUNOASSAY, 1983, 4 (03) :209-327

[6]

KAPLAN AP, 1987, BLOOD, V70, P1

[7] CONTINUOUS CULTURES OF FUSED CELLS SECRETING ANTIBODY OF PREDEFINED SPECIFICITY [J].

KOHLER, G ;

MILSTEIN, C .

NATURE, 1975, 256 (5517) :495-497

[8]

LACHMANN PJ, 1986, CIBA F SYMP, V119, P25

[9]

MANNHALTER C, 1987, Fibrinolysis, V1, P259, DOI 10.1016/0268-9499(87)90046-4

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