RNA interference: gene silencing in the fast lane

被引：46

作者：

Kittler, R ^{[1
]}

Buchholz, F ^{[1
]}

机构：

[1] Max Planck Inst Mol Cell Biol & Genet, D-01307 Dresden, Germany

来源：

SEMINARS IN CANCER BIOLOGY | 2003年 / 13卷 / 04期

关键词：

RNAi; siRNA; RNase III; gene silencing; whole embryo culture; DOUBLE-STRANDED-RNA; FUNCTIONAL GENOMIC ANALYSIS; MAMMALIAN-CELLS; C-ELEGANS; MOUSE MODELS; CAENORHABDITIS-ELEGANS; CARCINOMA CELLS; MESSENGER-RNA; HUMAN-DISEASE; HAIRPIN RNAS;

D O I：

10.1016/S1044-579X(03)00039-7

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Sequencing of whole genomes has provided new perspectives into the blueprints of diverse organisms. Knowing the sequences, however, does not always tell us much about the function of the genes that regulate development and homeostasis. RNA interference (RNAi) is becoming the method of choice for gene function analysis in cells and whole organisms. Here we review the approaches available to perform RNAi experiments in mammalian cells and in mice. We discuss usage of RNAi in cancer research and as a possible therapeutic tool for cancer treatment. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：259 / 265

页数：7

共 83 条

[1]

Gene centre chips in with better route to microarrays [J].

Abbott, A .

NATURE, 2002, 420 (6911) :3-3

[2]

Methyl-CpG binding domain protein 2 represses transcription from hypermethylated π-class glutathione S-transferase gene promoters in hepatocellular carcinoma cells [J].

Bakker, J ;

Lin, XH ;

Nelson, WG .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (25) :22573-22580

[3]

Retroviral delivery of small interfering RNA into primary cells [J].

Barton, GM ;

Medzhitov, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (23) :14943-14945

[4]

A SIMPLE AND EFFICIENT METHOD FOR DIRECT GENE DELETION IN SACCHAROMYCES-CEREVISIAE [J].

BAUDIN, A ;

OZIERKALOGEROPOULOS, O ;

DENOUEL, A ;

LACROUTE, F ;

CULLIN, C .

NUCLEIC ACIDS RESEARCH, 1993, 21 (14) :3329-3330

[5]

Mouse models of human disease .2. Recent progress and future directions [J].

Bedell, MA ;

Largaespada, DA ;

Jenkins, NA ;

Copeland, NG .

GENES & DEVELOPMENT, 1997, 11 (01) :11-43

[6]

Mouse models of human disease .1. Techniques and resources for genetic analysis in mice [J].

Bedell, MA ;

Jenkins, NA ;

Copeland, NG .

GENES & DEVELOPMENT, 1997, 11 (01) :1-10

[7]

Role for a bidentate ribonuclease in the initiation step of RNA interference [J].

Bernstein, E ;

Caudy, AA ;

Hammond, SM ;

Hannon, GJ .

NATURE, 2001, 409 (6818) :363-366

[8]

Comparison of antisense oligonucleotides and siRNAs in cell culture and in vivo [J].

Bertrand, JR ;

Pottier, M ;

Vekris, A ;

Opolon, P ;

Maksimenko, A ;

Malvy, C .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2002, 296 (04) :1000-1004

[9]

A system for stable expression of short interfering RNAs in mammalian cells [J].

Brummelkamp, TR ;

Bernards, R ;

Agami, R .

SCIENCE, 2002, 296 (5567) :550-553

[10]

The DT40 web site: sampling and connecting the genes of a B cell line [J].

Buerstedde, JM ;

Arakawa, H ;

Watahiki, A ;

Carninci, PP ;

Hayashizaki, YY ;

Korn, B ;

Plachy, J .

NUCLEIC ACIDS RESEARCH, 2002, 30 (01) :230-231

← 1 2 3 4 5 6 7 8 9 →