Biosynthesis of glucoamylase from Aspergillus niger by solid-state fermentation using tea waste as the basis of a solid substrate

被引:56
作者
Selvakumar, P [1 ]
Ashakumary, L [1 ]
Pandey, A [1 ]
机构
[1] CSIR, Reg Res Lab, Div Biotechnol, Trivandrum 695019, Kerala, India
关键词
tea waste; solid state fermentation; glucoamylase; Aspergillus niger;
D O I
10.1016/S0960-8524(98)00012-1
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Ten waste, supplemented with minerals, was used as the solid substrate for the synthesis of glucoamylase by a strain of Aspergillus niger NCIM 1248. Maximum glucoamylase synthesis [198.4 IU/g dry substrate (gds)] was obtained on the mineralised tea waste at 96 h with an inoculum, initial moisture and pH of 4%, 60% and 4.5, respectively at 30 degrees C. Among different carbon sources sucrose favoured the increase of glucoamylase synthesis (212.6 IU/gds) in 96 h, whereas among different nitrogen soul ces, malt extract gave the highest enzyme titre (226.0 IU/gds) at 72 h. (C) 1998 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:83 / 85
页数:3
相关论文
共 17 条
[1]  
Herbert D., 1971, METHODS MICROBIOL, V5, P209, DOI [10.1016/S0580-9517(08)70641-X, DOI 10.1016/S0580-9517(08)70641-X]
[2]   SOLID-STATE FERMENTATIONS [J].
HESSELTINE, CW .
BIOTECHNOLOGY AND BIOENGINEERING, 1972, 14 (04) :517-+
[3]  
HESSELTINE CW, 1987, INT BIODETERIOR, V23, P75
[4]  
LOWRY OH, 1951, J BIOL CHEM, V193, P265
[5]  
LULLA B. S., 1954, JOUR SCI AND INDUST RES [INDIA], V13B, P410
[6]  
Menon K. K. G., 1993, Tea, culture, processing and marketing, Coonoor, India, 23-24 Mar. 1992., P3
[7]   USE OF DINITROSALICYLIC ACID REAGENT FOR DETERMINATION OF REDUCING SUGAR [J].
MILLER, GL .
ANALYTICAL CHEMISTRY, 1959, 31 (03) :426-428
[8]  
Mudgetti R.E., 1986, Manual of Industrial Biotechnology, P66
[9]   THE PRODUCTION OF GLUCOAMYLASE BY ASPERGILLUS-NIGER NCIM-1245 [J].
PANDEY, A ;
RADHAKRISHNAN, S .
PROCESS BIOCHEMISTRY, 1993, 28 (05) :305-309
[10]   RECENT PROCESS-DEVELOPMENTS IN SOLID-STATE FERMENTATION [J].
PANDEY, A .
PROCESS BIOCHEMISTRY, 1992, 27 (02) :109-117