Efficient activation of viral genomes by levels of herpes simplex virus ICP0 insufficient to affect cellular gene expression or cell survival

被引:46
作者
Hobbs, WE
Brough, DE
Kovesdi, I
DeLuca, NA
机构
[1] Univ Pittsburgh, Sch Med, Dept Mol Genet & Biochem, Pittsburgh, PA 15261 USA
[2] GenVec Inc, Gaithersburg, MD 20878 USA
关键词
D O I
10.1128/JVI.75.7.3391-3403.2001
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Herpes simplex virus (HSV) ICP0 can effectively activate gene expression from otherwise silent promoters contained on persisting viral genomes. However, the expression of high levels of ICP0, as from ICP4(-) HSV type 1 (HSV-1) vectors, results in marked toxicity. We have analyzed the results of ICP0 expressed from an E1(-) E4(-) adenovirus vector (AdS.11E4ICP0) in which ICP0 expression is controlled from the endogenous adenoviral E4 promoter. In this system, the expression level of ICP0 was reduced more than 1,000-fold relative to the level of expression from HSV-1 vectors. This low level of ICP0 did not affect cellular division or greatly perturb cellular metabolism as assessed by gene expression array analysis comparing the effects of HSV and adenovirus vector strains. However, this amount of ICP0 was sufficient to quantitatively destroy ND10 structures as measured by promyelocytic leukemia immunofluorescence. The levels of adenovirus-expressed ICP0 were sufficient to activate quiescent viral genomes in trans and promote persistent transgene expression in cis. Moreover, infection of complementing cells with AdS.11E4ICP0 promoted viral growth and resulted in a 20-fold increase in the plaquing efficiency of d109, a virus defective for all five immediate-early genes. Thus, the low level expression of ICP0 from the E1(-) E4(-) adenovirus vector may increase the utility of adenovirus vectors and also provides a means to efficiently quantify and possibly propagate HSV vectors defective in ICP0. Importantly, the results demonstrate that the activation function of ICP0 may not result from changes in cellular gene expression, but possibly as a direct consequence of an enzymatic function inherent to the protein that may involve its action at ND10 resulting in the preferential activation of viral genomes.
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页码:3391 / 3403
页数:13
相关论文
共 60 条
[1]   The major immediate-early proteins IE1 and IE2 of human cytomegalovirus colocalize with and disrupt PML-asscciated nuclear bodies at very early times in infected permissive cells [J].
Ahn, JH ;
Hayward, GS .
JOURNAL OF VIROLOGY, 1997, 71 (06) :4599-4613
[2]   VITRONECTIN RECEPTOR ANTIBODIES INHIBIT INFECTION OF HELA AND A549 CELLS BY ADENOVIRUS-TYPE-12 BUT NOT BY ADENOVIRUS TYPE-2 [J].
BAI, M ;
CAMPISI, L ;
FREIMUTH, P .
JOURNAL OF VIROLOGY, 1994, 68 (09) :5925-5932
[3]   THE HERPES-SIMPLEX VIRUS TYPE-1 REGULATORY PROTEIN ICP0 ENHANCES VIRUS-REPLICATION DURING ACUTE INFECTION AND REACTIVATION FROM LATENCY [J].
CAI, WH ;
ASTOR, TL ;
LIPTAK, LM ;
CHO, C ;
COEN, DM ;
SCHAFFER, PA .
JOURNAL OF VIROLOGY, 1993, 67 (12) :7501-7512
[4]   TARGETING OF ADENOVIRUS E1A AND E4-ORF3 PROTEINS TO NUCLEAR MATRIX-ASSOCIATED PML BODIES [J].
CARVALHO, T ;
SEELER, JS ;
OHMAN, K ;
JORDAN, P ;
PETTERSSON, U ;
AKUSJARVI, G ;
CARMOFONSECA, M ;
DEJEAN, A .
JOURNAL OF CELL BIOLOGY, 1995, 131 (01) :45-56
[5]  
ChelbiAlix MK, 1995, LEUKEMIA, V9, P2027
[6]   HERPES-SIMPLEX VIRUSES WITH MUTATIONS IN THE GENE ENCODING ICP0 ARE DEFECTIVE IN GENE-EXPRESSION [J].
CHEN, JX ;
SILVERSTEIN, S .
JOURNAL OF VIROLOGY, 1992, 66 (05) :2916-2927
[7]   A HERPES-SIMPLEX VIRUS TYPE-1 MUTANT CONTAINING A DELETION WITHIN IMMEDIATE EARLY GENE-1 IS LATENCY-COMPETENT IN MICE [J].
CLEMENTS, GB ;
STOW, ND .
JOURNAL OF GENERAL VIROLOGY, 1989, 70 :2501-2506
[8]   ISOLATION AND CHARACTERIZATION OF DELETION MUTANTS OF HERPES-SIMPLEX VIRUS TYPE-1 IN THE GENE ENCODING IMMEDIATE-EARLY REGULATORY PROTEIN-ICP4 [J].
DELUCA, NA ;
MCCARTHY, AM ;
SCHAFFER, PA .
JOURNAL OF VIROLOGY, 1985, 56 (02) :558-570
[9]   ACTIVITIES OF HERPES-SIMPLEX VIRUS TYPE-1 (HSV-1) ICP4 GENES SPECIFYING NONSENSE PEPTIDES [J].
DELUCA, NA ;
SCHAFFER, PA .
NUCLEIC ACIDS RESEARCH, 1987, 15 (11) :4491-4511
[10]   POINT MUTATIONS IN THE HERPES-SIMPLEX VIRUS TYPE-1 VMW110 RING FINGER HELIX AFFECT ACTIVATION OF GENE-EXPRESSION, VIRAL GROWTH, AND INTERACTION WITH PML-CONTAINING NUCLEAR-STRUCTURES [J].
EVERETT, R ;
OHARE, P ;
OROURKE, D ;
BARLOW, P ;
ORR, A .
JOURNAL OF VIROLOGY, 1995, 69 (11) :7339-7344