Second-generation ethanol production from steam exploded barley straw by Kluyveromyces marxianus CECT 10875

被引：61

作者：

Garcia-Aparicio, M. P. ^{[1
]}

Oliva, J. M. ^{[1
]}

Manzanares, P. ^{[1
]}

Ballesteros, M. ^{[1
]}

Ballesteros, I. ^{[1
]}

Gonzalez, A. ^{[1
]}

Negro, M. J. ^{[1
]}

机构：

[1] CIEMAT, Biofuels Unit, Renewable Energies Dept, E-28040 Madrid, Spain

来源：

FUEL | 2011年 / 90卷 / 04期

关键词：

Ethanol; Barley straw; Steam explosion pretreatment; SSF; Thermotolerant yeast; PRETREATED CORN STOVER; DRY-MATTER CONTENT; FED-BATCH PROCESS; SIMULTANEOUS SACCHARIFICATION; ENZYMATIC-HYDROLYSIS; BIOETHANOL PRODUCTION; WHEAT-STRAW; EXPLOSION PRETREATMENT; CELLULOSE HYDROLYSIS; FERMENTATION;

D O I：

10.1016/j.fuel.2010.10.052

中图分类号：

TE [石油、天然气工业]; TK [能源与动力工程];

学科分类号：

0807 ; 0820 ;

摘要：

Barley straw is nowadays being considered a potential lignocellulosic raw material for fuel-ethanol production as an alternative to starch-or sugar-containing feedstock. In this work, several configuration strategies for ethanol production from steam-exploded barley straw by Kluyveromyces marxianus CECT 10875 have been studied with the aim of obtaining higher ethanol concentrations. Different substrate loading (2-15%, w/v) were studied during enzymatic hydrolysis. The xylanase contribution on glucose production and glucan conversion at different substrate loading was also investigated. In addition, three different process configurations, separate hydrolysis and fermentation, simultaneous saccharification and fermentation and presaccharification and simultaneous saccharification, were compared at different water insoluble solids concentration (5%, 10% and 15%). The influence of xylanase addition on the ethanol yield was studied as well. Results show that endo-xylanases improved glucan conversion and ethanol yield compared with a standard enzymatic mixture, markedly at low substrate concentration. The positive effect of added xylanase was most evident at early stages of enzymatic hydrolysis. Regarding process configurations for the period of 72 h, SSF with endo-xylanases provided the best ethanol yield, nearly 70%, for 10% WIS. Nonetheless, the higher ethanol concentration, 29.4 g/l, was obtained at 15% WIS. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：1624 / 1630

页数：7

共 42 条

[21] Effect of xylanase supplementation of cellulase on digestion of corn stover solids prepared by leading pretreatment technologies [J].

Kumar, Rajeev ;

Wyman, Charles E. .

BIORESOURCE TECHNOLOGY, 2009, 100 (18) :4203-4213

[22]

Lane Melanie M., 2010, Fungal Biology Reviews, V24, P17, DOI 10.1016/j.fbr.2010.01.001

[23] Simultaneous saccharification and fermentation of steam-pretreated barley straw at low enzyme loadings and low yeast concentration [J].

Linde, M. ;

Galbe, M. ;

Zacchi, G. .

ENZYME AND MICROBIAL TECHNOLOGY, 2007, 40 (05) :1100-1107

[24] Substrate and enzyme characteristics that limit cellulose hydrolysis [J].

Mansfield, SD ;

Mooney, C ;

Saddler, JN .

BIOTECHNOLOGY PROGRESS, 1999, 15 (05) :804-816

[25] Effect of hemicellulose and lignin removal on enzymatic hydrolysis of steam pretreated corn stover [J].

Ohgren, Karin ;

Bura, Renata ;

Saddler, Jack ;

Zacchi, Guido .

BIORESOURCE TECHNOLOGY, 2007, 98 (13) :2503-2510

[26] A comparison between simultaneous saccharification and fermentation and separate hydrolysis and fermentation using steam-pretreated corn stover [J].

Ohgren, Karin ;

Bura, Renata ;

Lesnicki, Gary ;

Saddler, Jack ;

Zacchi, Guido .

PROCESS BIOCHEMISTRY, 2007, 42 (05) :834-839

[27] High temperature enzymatic prehydrolysis prior to simultaneous saccharification and fermentation of steam pretreated corn stover for ethanol production [J].

Ohgren, Karin ;

Vehmaanpera, Jari ;

Siika-Aho, Matti ;

Galbe, Mats ;

Viikari, Liisa ;

Zacchi, Guido .

ENZYME AND MICROBIAL TECHNOLOGY, 2007, 40 (04) :607-613

[28] Effect of lignocellulosic degradation compounds from steam explosion pretreatment on ethanol fermentation by thermotolerant yeast Kluyveromyces marxianus [J].

Oliva, JM ;

Sáez, F ;

Ballesteros, I ;

González, A ;

Negro, MJ ;

Manzanares, P ;

Ballesteros, M .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2003, 105 (1-3) :141-153

[29] A short review on SSF - an interesting process option for ethanol production from lignocellulosic feedstocks [J].

Olofsson, Kim ;

Bertilsson, Magnus ;

Liden, Gunnar .

BIOTECHNOLOGY FOR BIOFUELS, 2008, 1 (1)

[30] Effect of inhibitors released during steam-explosion pretreatment of barley straw on enzymatic hydrolysis [J].

Prado Garcia-Aparicio, M. ;

Ballesteros, Ignacio ;

Gonzalez, Alberto ;

Miguel Oliva, Jose ;

Ballesteros, Mercedes ;

Jose Negro, M. .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2006, 129 (1-3) :278-288

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