Polar amino acid-rich sequences bind to polyglutamine tracts

被引：37

作者：

Imafuku, I

Waragai, M

Takeuchi, S

Kanazawa, I

Kawabata, M

Mouradian, MM

Okazawa, H

机构：

[1] Univ Tokyo, Grad Sch Med, Dept Neurol, Grp Mol Neurobiol,Bunkyo Ku, Tokyo 113, Japan

[2] Japanese Fdn Canc Res, Inst Canc, Dept Biochem, Toshima Ku, Tokyo 170, Japan

[3] NINDS, Genet Pharmacol Unit, Expt Therapeut Branch, NIH, Bethesda, MD 20892 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 253卷 / 01期

关键词：

D O I：

10.1006/bbrc.1998.9725

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Polyglutamine tracts are found in different proteins including transcription factors and cofactors as well as in triplet repeat disease gene products. To characterize the protein motif that binds to the polyglutamine tract, we screened a human embryonic brain cDNA library with the polyglutamine tract of Brn-2 as bait using the yeast two-hybrid method. All six isolated clones encoding polyglutamine tract binding proteins were rich in polar amino acids. Three of these clones could form polar helical structures. These observations suggest that polar amino acid-rich sequences are essential for binding to the polyglutamine tract. (C) 1998 Academic Press.

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页码：16 / 20

页数：5

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