Functional characterization of an ammonium transporter gene from Lotus japonicus

被引：54

作者：

Salvemini, F

Marini, AM

Riccio, A

Patriarca, EJ

Chiurazzi, M

机构：

[1] Int Inst Genet & Biophys, I-80125 Naples, Italy

[2] Univ Libre Bruxelles, IBMM, B-6041 Gosselies, Belgium

来源：

GENE | 2001年 / 270卷 / 1-2期

关键词：

intron; functional complementation; symbiosis;

D O I：

10.1016/S0378-1119(01)00470-X

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

NH4+ is the main product of symbiotic nitrogen fixation and the external concentration of combined nitrogen plays a key regulatory role in all the different step of plant-rhizobia interaction. We report the cloning and characterization of the first member of the ammonium transporter family, LjAMT1:1 from a leguminous plant, Lotus japonicus. Sequence analysis reveals a close relationship to plant transporters of the AMT1 family. The wild type and two mutated versions of LjAMT1:1 were expressed and functionally characterized in yeast. LjAMT1:1 is transcribed in roots, leaves and nodules of L. japonicus plants grown under low nitrogen conditions. consistent with a role in uptake of NH4+ by the plant cells. (C) 2001 Elsevier Science B.V. All rights reserved.

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页码：237 / 243

页数：7

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