Measurement of biodegradable substances using the salt-tolerant yeast Arxula adeninivorans for a microbial sensor immobilized with poly(carbamoyl) sulfonate (PCS) part I:: Construction and characterization of the microbial sensor

被引：46

作者：

Chan, CY

Lehmann, M

Tag, K

Lung, M

Kunze, G

Riedel, K

Gruendig, B

Renneberg, R

机构：

[1] Hong Kong Univ Sci & Technol, Dept Chem, Hong Kong, Peoples R China

[2] Dept Chem Engn Biosensor, Hong Kong, Peoples R China

[3] Bioelect Lab, Hong Kong, Peoples R China

[4] Inst Plant Genet, D-06466 Gatersleben, Germany

[5] Crop Plant Res, D-06466 Gatersleben, Germany

[6] Hong Kong Univ Sci & Technol, Dept Biol, Hong Kong, Peoples R China

[7] Dr Bruno Lange GmbH Berlin, D-40549 Duesseldorf, Germany

[8] SensLab, D-04318 Leipzig, Germany

来源：

BIOSENSORS & BIOELECTRONICS | 1999年 / 14卷 / 02期

关键词：

microbial sensor; biodegradable substances; Arxula;

D O I：

10.1016/S0956-5663(98)00110-9

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A microbial biosensor based on the yeast Arxula adeninivorans LS3 has been developed for measurement of biodegradable substances. Arxula is immobilized in the hydrogel poly(carbamoyl) sulfonate (PCS). The immobilized yeast membrane is placed in front of an oxygen electrode with - 600 mV versus Ag/AgCl. Arxula is salt tolerant; it can give a stable signal up to 2.5 M NaCl in sample (120 mM in measuring cell). The sensor's measurements are highly correlated to BOD5 measurements. It has a very high stability which can last for 40 days without any decrease in signal. The linear range of the sensor is up to a corresponding BOD value of 550 mg/l. (C) 1999 Elsevier Science S.A. All rights reserved.

引用

页码：131 / 138

页数：8

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