A statistical analysis of N- and O-glycan linkage conformations from crystallographic data

被引:104
作者
Petrescu, AJ
Petrescu, SM
Dwek, RA
Wormald, MR
机构
[1] Univ Oxford, Dept Biochem, Oxford Glycobiol Inst, Oxford OX1 3QU, England
[2] Romanian Acad, Inst Biochem, Bucharest 77700 17, Romania
基金
英国惠康基金;
关键词
oligosaccharide; linkage conformers; linkage distortion;
D O I
10.1093/glycob/9.4.343
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have generated a database of 639 glycosidic linkage structures by an exhaustive survey of the available crystallographic data for isolated oligosaccharides, glycoproteins, and glycan-binding proteins. For isolated oligosaccharides there is relatively little crystallographic data available, A much larger number of glycoprotein and glycan-binding protein structures have now been solved in which two or more linked monosaccharides can be resolved. In the majority of these cases, only a few residues can be seen. Using the 639 glycosidic linkage structures, we have identified one or more distinct conformers for all the linkages, The O5-C1-O-C(x)' torsion angles for all these distinct conformers appear to be determined chiefly by the exo-anomeric effect. The Man alpha 1-6Man linkage appears to be less restrained than the others, showing a wide degree of dispersion outside the ranges of the defined conformers. The identification of distinct conformers for glycosidic linkages allows "average" glycan structures to be modeled and also allows the easy identification of distorted glycosidic linkages; Such an analysis shows that the interactions between IgG Fc and its own N-linked glycan result in severe distortion of the terminal Gal beta 1-4GlcNAc linkage only, indicating the strong interactions that must be present between the Gal residue and the protein surface. The applicability of this crystallographic based analysis to glycan structures in solution is discussed. This database of linkage structures should be a very useful reference tool in three-dimensional structure determinations.
引用
收藏
页码:343 / 352
页数:10
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