The effect of incorporating RGD adhesive peptide in polyethylene glycol diacrylate hydrogel on osteogenesis of bone marrow stromal cells

被引：375

作者：

Yang, F ^{[1
]}

Williams, CG ^{[1
]}

Wang, DA ^{[1
]}

Lee, H ^{[1
]}

Manson, PN ^{[1
]}

Elisseeff, J ^{[1
]}

机构：

[1] Johns Hopkins Univ, Dept Biomed Engn, Baltimore, MD 21218 USA

来源：

BIOMATERIALS | 2005年 / 26卷 / 30期

基金：

美国国家卫生研究院;

关键词：

RGD; marrow stromal cells; osteogenesis; photopolymerizing; hydrogel;

D O I：

10.1016/j.biomaterials.2005.03.018

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Advances in tissue engineering require biofunctional scaffolds that can. not only provide cells with structural support, but also interact with cells in a biological manner. To achieve this goal, a frequently used cell adhesion peptide Arg-Gly-Asp (RGD) was covalently incorporated into poly(ethylene glycol) diacrylate (PEODA) h, droget and its dosage effect (0.025, 1.25 and 2.5 mm) on osteogenesis of marrow stromal cells in a three-dimensional environment was examined. Expression of bone-related markers, osteocalcin (OCN) and Alkaline phosphatase (ALP), increased significantly as the RGD concentration increased. Compared with no RGD, 2.5mM RGD group showed a 1344% increase in ALP production and a 277% increase in OCN accumulation in the medium. RGD helped MSCs maintain cbfa-1 expression when shifted from a two-dimensional environment to a three-dimensional environment. Soluble RGD was found to completely block the mineralization of marrow stromal cells, as manifested by quantitative calcium assay, phosphorus elemental analysis and Von Kossa staining. In conclusion, we have demonstrated that RGD-conjugated PEODA hydrogel promotes the osteogenesis of MSCs in a dosage-dependent manner, with 2.5 mm being optimal concentration. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：5991 / 5998

页数：8

共 22 条

[1] Cell-interactive alginate hydrogels for bone tissue engineering [J].

Alsberg, E ;

Anderson, KW ;

Albeiruti, A ;

Franceschi, RT ;

Mooney, DJ .

JOURNAL OF DENTAL RESEARCH, 2001, 80 (11) :2025-2029

[2]

Bryant S J, 1999, Biomed Sci Instrum, V35, P309

[3] Photoencapsulation of osteoblasts in injectable RGD-modified PEG hydrogels for bone tissue engineering [J].

Burdick, JA ;

Anseth, KS .

BIOMATERIALS, 2002, 23 (22) :4315-4323

[4] Transdermal photopolymerization for minimally invasive implantation [J].

Elisseeff, J ;

Anseth, K ;

Sims, D ;

McIntosh, W ;

Randolph, M ;

Langer, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (06) :3104-3107

[5]

Hoffman AS, 2001, ANN NY ACAD SCI, V944, P62

[6]

Huang Hui, 2003, J Oral Implantol, V29, P73, DOI 10.1563/1548-1336(2003)029<0073:EOFORI>2.3.CO

[7]

[8] Enhancement of osteogenesis on hydroxyapatite surface coated with synthetic peptide (EEEEEEEPRGDT) in vitro [J].

Itoh, D ;

Yoneda, S ;

Kuroda, S ;

Kondo, H ;

Umezawa, A ;

Ohya, K ;

Ohyama, T ;

Kasugai, S .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 2002, 62 (02) :292-298

[9] TISSUE ENGINEERING [J].

LANGER, R ;

VACANTI, JP .

SCIENCE, 1993, 260 (5110) :920-926

[10] Modification of surfaces with cell adhesion peptides alters extracellular matrix deposition [J].

Mann, BK ;

Tsai, AT ;

Scott-Burden, T ;

West, JL .

BIOMATERIALS, 1999, 20 (23-24) :2281-2286

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