A Ligation and photorelease strategy for the temporal and spatial control of protein function in living cells

被引：47

作者：

Pellois, JP ^{[1
]}

Muir, TW ^{[1
]}

机构：

[1] Rockefeller Univ, Lab Synth Prot Chem, New York, NY 10021 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 35期

关键词：

bioorganic chemistry; drug delivery; photoactivation; proteins;

D O I：

10.1002/anie.200501244

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Graph Presented) Design and light: A photocleavable synthetic moiety is ligated to the C terminus of a recombinant protein. In the ligated state, the chemical and protein components influence the properties of one another. Upon irradiation with light, both elements are released and their respective function is recovered. In this way a protein can be delivered into living cells and activated by UV irradiation at a desired time or location. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：5713 / 5717

页数：5

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