Calmodulin binding to the polybasic C-termini of STIM proteins involved in store-operated calcium entry

被引：52

作者：

Bauer, Mikael C. ^{[1
]}

O'Connell, David ^{[2
]}

Cahill, Dolores J. ^{[2
]}

Linse, Sara ^{[1
,2
]}

机构：

[1] Lund Univ, Ctr Chem, SE-22100 Lund, Sweden

[2] Univ Coll Dublin, Conway Inst Biomol & Biomed Res, Dublin 4, Ireland

来源：

BIOCHEMISTRY | 2008年 / 47卷 / 23期

关键词：

D O I：

10.1021/bi800496a

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Translocation of STIM1 and STIM2 from the endoplasmic reticulum to the plasma membrane is a key step in store-operated calcium entry in the cell. We show by isothermal titration calorimetry that calmodulin binds in a calcium-dependent manner to the polybasic C-termini of STIM1 and STIM2, a region critical for their translocation to the plasma membrane (K-D <= 1 mu M in calcium). HSQC NMR spectroscopy shows this interaction is in the fast exchange regime. By binding STIM1 and STIM2, calmodulin may regulate store refilling, thereby ensuring the maintenance of its own action in intracellular signaling.

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收藏

页码：6089 / 6091

页数：3

相关论文

共 23 条

[1]

Salt enhances calmodulin-target interaction [J].

André, I ;

Kesvatera, T ;

Jönsson, B ;

Linse, S .

BIOPHYSICAL JOURNAL, 2006, 90 (08) :2903-2910

[2]

The role of electrostatic interactions in calmodulin-peptide complex formation [J].

André, I ;

Kesvatera, T ;

Jönsson, B ;

Åkerfeldt, KS ;

Linse, S .

BIOPHYSICAL JOURNAL, 2004, 87 (03) :1929-1938

[3]

140 Mouse brain proteins identified by Ca2+-calmodulin affinity chromatography and tandem mass spectrometry [J].

Berggård, T ;

Arrigoni, G ;

Olsson, O ;

Fex, M ;

Linse, S ;

James, P .

JOURNAL OF PROTEOME RESEARCH, 2006, 5 (03) :669-687

[4]

STIM2 is a feedback regulator that stabilizes basal cytosolic and endoplasmic reticulum Ca2+ levels [J].

Brandman, Onn ;

Liou, Jen ;

Park, Wei Sun ;

Meyer, Tobias .

CELL, 2007, 131 (07) :1327-1339

[5]

MOLECULAR AND STRUCTURAL BASIS OF TARGET RECOGNITION BY CALMODULIN [J].

CRIVICI, A ;

IKURA, M .

ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE, 1995, 24 :85-116

[6]

Calmodulin in action: Diversity in target recognition and activation mechanisms [J].

Hoeflich, KP ;

Ikura, M .

CELL, 2002, 108 (06) :739-742

[7]

STIM1 carboxyl-terminus activates native SOC, Icrac and TRPC1 channels [J].

Huang, Guo N. ;

Zeng, Weizhong ;

Kim, Joo Young ;

Yuan, Joseph P. ;

Han, Linhuang ;

Muallem, Shmuel ;

Worley, Paul F. .

NATURE CELL BIOLOGY, 2006, 8 (09) :1003-U96

[8]

Genetic polymorphism and protein conformational plasticity in the calmodulin superfamily: Two ways to promote multifunctionality [J].

Ikura, M ;

Ames, JB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (05) :1159-1164

[9]

SOLUTION STRUCTURE OF A CALMODULIN-TARGET PEPTIDE COMPLEX BY MULTIDIMENSIONAL NMR [J].

IKURA, M ;

CLORE, GM ;

GRONENBORN, AM ;

ZHU, G ;

KLEE, CB ;

BAX, A .

SCIENCE, 1992, 256 (5057) :632-638

[10]

Mapping the interacting domains of STIM1 and Orai1 in Ca2+ release-activated Ca2+ channel activation [J].

Li, Zhengzheng ;

Lu, Jingze ;

Xu, Pingyong ;

Xie, Xiangyang ;

Chen, Liangyi ;

Xu, Tao .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2007, 282 (40) :29448-29456