Investigation of Soy Protein-based Biostimulant Seed Coating for Broccoli Seedling and Plant Growth Enhancement

This research presents a novel method of using plant-derived protein hydrolysates as seed coating materials. The objective of this study was to develop seed coating formulations using soy flour, a sustainable, inexpensive, and green source, as a biostimulant using broccoli as the model system. A 10% suspension of soy flour was used as the seed treatment binder in all coatings. The solid particulate filler was composed of mixtures of soy flour, cellulose, and diatomaceous earth, together termed as SCD. All SCD components were homogenized in water, then dried and ground to a fine particle size <106 mu m. The SCD coatings were applied with rotary pan seed coating equipment at 25% of the seed weight. Increasing the proportion of soy flour increased the seed coating strength and also the time for the coating to disintegrate after soaking in water. As a result, the seed coatings reduced the percentage germination and the germination rate compared with the nontreated control. However, the 10-day-old seedling root and shoot growth showed significant improvement for all SCD coating treatments compared with controls. Plant growth and development was also measured after 30 days in the greenhouse. Fresh weight (FW) and dry weight (DW), leaf area, plant height, leaf development, Soil-Plant Analyses Development (SPAD) index (chlorophyll measurement), and nitrogen (N) per plant were all greater from coatings with 30%, 40%, and 50% soy flour than the noncoated control. Nitrogen, from the soy flour applied in the seed coatings, ranged from 0.024 to 0.073 mg per seed, while the enhanced N per plant ranged from 1.7 to 8.5 mg. The coating treatment with 0.063 mg N per seed resulted in the greatest plant leaf area and highest N content. Nitrogen applied in the seed coating only accounted for 1% to 2% of the enhanced N in the plants, indicating the soy flour acted as a biostimulant rather than a fertilizer.