The effects of selected cotton-leaf volatiles on growth, development and aflatoxin production of Aspergillus parasiticus

被引：38

作者：

Greene-McDowelle, DM ^{[1
]}

Ingber, B ^{[1
]}

Wright, MS ^{[1
]}

Zeringue, HJ ^{[1
]}

Bhatnagar, D ^{[1
]}

Cleveland, TE ^{[1
]}

机构：

[1] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA

来源：

TOXICON | 1999年 / 37卷 / 06期

关键词：

Aspergillus parasiticus; cotton-leaf; volatiles; aerial hyphae;

D O I：

10.1016/S0041-0101(98)00209-8

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The fungi Aspergillus flavus and Aspergillus parasiticus produce the hepatocarcinogenic, secondary metabolites, aflatoxins, in cottonseed, corn, peanuts and treenuts. Results have shown that aflatoxigenic strains of A. flavus and A. parasiticus grown in the presence of specific cotton-leaf volatiles exhibit alterations in aflatoxin production accompanied by variations in growth of the fungi. In this study, two alcohols (3-methyl-1-butanol (3-MB) and nonanol) and two terpenes (camphene and limonene) were chosen as representative cotton-leaf volatiles based on the effects they had on fungal growth and/ or aflatoxin production in previous investigations. The morphological effects of volatile exposure were examined in correlation with fungal growth and aflatoxin production. 3-MB-treated samples exhibited a decrease in fungal radial growth which was directly proportional to the volatile dosage. Additionally, 3-MB treatment resulted in loss of mycelial pigmentation and a decrease in sporulation. Limonene and camphene-treated samples yielded negligible differences in radial growth and morphology when compared to unexposed controls. In addition to radial growth inhibition, samples grown in the presence of nonanol demonstrated uniquely aerial hyphae. In comparison to an unexposed control, aflatoxin production increased in cultures exposed to 3-MB but decreased when exposed to the other three volatiles studied. Published by Elsevier Science Ltd.

引用

页码：883 / 893

页数：11

共 18 条

[1] INCORPORATION OF LABELLED COMPOUNDS INTO AFLATOXINS [J].

ADYE, J ;

MATELES, RI .

BIOCHIMICA ET BIOPHYSICA ACTA, 1964, 86 (02) :418-&

[2]

BENNETT JW, 1994, FEMS SYMP, P51

[3] ENZYMOLOGICAL EVIDENCE FOR SEPARATE PATHWAYS FOR AFLATOXIN-B1 AND AFLATOXIN-B2 BIOSYNTHESIS [J].

BHATNAGAR, D ;

CLEVELAND, TE ;

KINGSTON, DGI .

BIOCHEMISTRY, 1991, 30 (17) :4343-4350

[4] IDENTIFICATION OF O-METHYLSTERIGMATOCYSTIN AS AN AFLATOXIN-B1 AND AFLATOXIN-G1 PRECURSOR IN ASPERGILLUS-PARASITICUS [J].

BHATNAGAR, D ;

MCCORMICK, SP ;

LEE, LS ;

HILL, RA .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1987, 53 (05) :1028-1033

[5] THE INHIBITORY EFFECT OF NEEM (AZADIRACHTA-INDICA) LEAF EXTRACTS ON AFLATOXIN SYNTHESIS IN ASPERGILLUS-PARASITICUS [J].

BHATNAGAR, D ;

MCCORMICK, SP .

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1988, 65 (07) :1166-1168

[6]

BHATNAGAR D, 1990, NEEMS POTENTIAL PEST, P118

[7] CLONING OF THE ASPERGILLUS-PARASITICUS APA-2 GENE ASSOCIATED WITH THE REGULATION OF AFLATOXIN BIOSYNTHESIS [J].

CHANG, PK ;

CARY, JW ;

BHATNAGAR, D ;

CLEVELAND, TE ;

BENNETT, JW ;

LINZ, JE ;

WOLOSHUK, CP ;

PAYNE, GA .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (10) :3273-3279

[8] MYCOTOXINS - FOOD CONTAMINATION, MECHANISM, CARCINOGENIC POTENTIAL AND PREVENTIVE MEASURES [J].

CHU, FS .

MUTATION RESEARCH, 1991, 259 (3-4) :291-306

[9] EFFECTS OF CULTIVAR AND BOLL AGE ON AFLATOXIN IN COTTONSEED AFTER INOCULATION WITH ASPERGILLUS-FLAVUS AT SIMULATED EXIT HOLES OF THE PINK-BOLLWORM [J].

COTTY, PJ .

PLANT DISEASE, 1989, 73 (06) :489-492

[10]

Council for Agricultural Science and Technology (CAST), 1989, 116 CAST

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