Direct immunodetection of antigens within the precast polyacrylamide gel

被引：11

作者：

Desai, S ^{[1
]}

Dworecki, B ^{[1
]}

Cichon, E ^{[1
]}

机构：

[1] Pierce Chem Co, Rockford, IL 61105 USA

来源：

ANALYTICAL BIOCHEMISTRY | 2001年 / 297卷 / 01期

关键词：

in-gel immunodetection; SDS-PAGE; Western blot; polyacrylamide gels; chemiluminescent substrate; electrophoresis;

D O I：

10.1006/abio.2001.5324

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Detection of specific proteins separated by SDS-PAGE is the basis for studying specific antigens. Immunodetection of antigens is commonly performed using Western blotting technique. In this paper we have shown that it is possible to eliminate Western blotting and to detect the antigens directly within the precast polyacrylamide gels by pretreating the gels with 50% isopropanol followed by distilled water treatment. This method would be valuable for large or difficult to transfer proteins. (C) 2001 Academic Press.

引用

页码：94 / 98

页数：5

共 11 条

[1] LOCALIZATION OF CELLULAR ANTIGENS IN SODIUM DODECYL SULFATE-POLYACRYLAMIDE GELS [J].