Protection against mucosal SIVsm challenge in macaques infected with a chimeric SIV that expresses HIV type 1 envelope

被引:54
作者
QuesadaRolander, M
Makitalo, B
Thorstensson, R
Zhang, YJ
CastanosVelez, E
Biberfeld, G
Putkonen, P
机构
[1] KAROLINSKA INST,CTR MICROBIOL & TUMOR BIOL,S-17177 STOCKHOLM,SWEDEN
[2] KAROLINSKA INST,IMMUNOPATHOL LAB,S-10401 STOCKHOLM,SWEDEN
关键词
D O I
10.1089/aid.1996.12.993
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
In a monkey model we used a chimeric SIV expressing the HIV-1 envelope gene (SHIV-4) as a live attenuated vaccine and a virulent SIVsm as a mucosal challenge, Four cynomolgus monkeys were inoculated intravenously with SHIV-4, Virus was repeatedly isolated from blood mononuclear cells of all four animals for 2 to 7 months after the inoculation of SHIV, All monkeys developed neutralizing antibodies to HIV-1 and high antibody titers to HIV-1 envelope glycoproteins. III contrast, no neutralizing antibodies to SIVsm were detected and cross-reacting antibodies to SIV envelope glycoproteins were demonstrable in low titers, Nine to 12 months after the SHIV inoculation the four monkeys and six naive control monkeys were challenged intrarectally with 10 monkey infectious doses of macaque cell-grown SIVsm. After a follow-up period of 1 year, two of four SHIV-infected monkeys were completely protected against SIVsm infection as shown by repeated negative virus isolations and negative polymerase chain reaction for SIV envelope DNA, One naive monkey that received blood from the two protected monkeys showed no signs of infection, The remaining two SHIV-infected monkeys showed an initial infection on challenge with SIVsm, but viral replication was thereafter suppressed. Cytotoxic T lymphocytes to SIV Nef and RT were demonstrable in one of four SHIV-infected monkeys before SIVsm challenge, but this monkey was not protected against SN infection, All six control animals yielded virus repeatedly after SIVsm challenge and three of them showed declining CD4 cell counts, Thus, infection with SHIV expressing HIV-1 envelope could induce cross-protection against mucosal SIVsm challenge.
引用
收藏
页码:993 / 999
页数:7
相关论文
共 27 条
  • [1] PROTECTION BY ATTENUATED SIMIAN IMMUNODEFICIENCY VIRUS IN MACAQUES AGAINST CHALLENGE WITH VIRUS-INFECTED CELLS
    ALMOND, N
    KENT, K
    CRANAGE, M
    RUD, E
    CLARKE, B
    STOTT, EJ
    [J]. LANCET, 1995, 345 (8961): : 1342 - 1344
  • [2] TRANSMISSION OF HTLV-III INFECTION FROM HUMAN-PLASMA TO CHIMPANZEES - AN ANIMAL-MODEL FOR AIDS
    ALTER, HJ
    EICHBERG, JW
    MASUR, H
    SAXINGER, WC
    GALLO, R
    MACHER, AM
    LANE, HC
    FAUCI, AS
    [J]. SCIENCE, 1984, 226 (4674) : 549 - 552
  • [3] ANDERSSON S, 1996, UNPUB IMMUNOGENICITY
  • [4] PROTECTION OF CHIMPANZEES FROM INFECTION BY HIV-1 AFTER VACCINATION WITH RECOMBINANT GLYCOPROTEIN GP120 BUT NOT GP160
    BERMAN, PW
    GREGORY, TJ
    RIDDLE, L
    NAKAMURA, GR
    CHAMPE, MA
    PORTER, JP
    WURM, FM
    HERSHBERG, RD
    COBB, EK
    EICHBERG, JW
    [J]. NATURE, 1990, 345 (6276) : 622 - 625
  • [5] BIBERFELD G, 1988, AIDS, V2, P195
  • [6] EARLY DIAGNOSIS OF HIV-1 INFECTION IN INFANTS IN DAR-ES-SALAAM, TANZANIA
    BREDBERGRADEN, U
    URASSA, E
    GRANKVIST, O
    MASSAWE, A
    LYAMUYA, E
    KAWO, G
    MSEMO, G
    KAZIMOTO, T
    MGONE, J
    MBENA, E
    KARLSSON, K
    MHALU, F
    BIBERFELD, G
    [J]. CLINICAL AND DIAGNOSTIC VIROLOGY, 1995, 4 (02): : 163 - 173
  • [7] THE SIMIAN IMMUNODEFICIENCY VIRUSES
    DESROSIERS, RC
    [J]. ANNUAL REVIEW OF IMMUNOLOGY, 1990, 8 : 557 - 578
  • [8] ISOLATION OF A T-LYMPHOTROPIC RETROVIRUS FROM NATURALLY INFECTED SOOTY MANGABEY MONKEYS (CERCOCEBUS-ATYS)
    FULTZ, PN
    MCCLURE, HM
    ANDERSON, DC
    SWENSON, RB
    ANAND, R
    SRINIVASAN, A
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (14) : 5286 - 5290
  • [9] EARLY SUPPRESSION OF SIV REPLICATION BY CD8+ NEF-SPECIFIC CYTOTOXIC T-CELLS IN VACCINATED MACAQUES
    GALLIMORE, A
    CRANAGE, M
    COOK, N
    ALMOND, N
    BOOTMAN, J
    RUD, E
    SILVERA, P
    DENNIS, M
    CORCORAN, T
    STOTT, J
    MCMICHAEL, A
    GOTCH, F
    [J]. NATURE MEDICINE, 1995, 1 (11) : 1167 - 1173
  • [10] GOTCH F, 1993, J MED PRIMATOL, V22, P119