Preparation and chemical and biological characterization of a pectin/chitosan polyelectrolyte complex scaffold for possible bone tissue engineering applications

被引：158

作者：

Coimbra, P. ^{[1
]}

Ferreira, P. ^{[1
,2
]}

de Sousa, H. C. ^{[1
]}

Batista, P. ^{[3
]}

Rodrigues, M. A. ^{[3
]}

Corriea, I. J. ^{[3
]}

Gil, M. H. ^{[1
]}

机构：

[1] Univ Coimbra, CIEPQPF, Dept Engn Quim, P-3030290 Coimbra, Portugal

[2] Univ Catolica Portuguesa, ICS, P-3504505 Viseu, Portugal

[3] Univ Beira Interior, Ctr Invest Ciencias Saude, Fac Ciencias Saude, P-6200506 Covilha, Portugal

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2011年 / 48卷 / 01期

关键词：

Polymeric scaffold; Polyelectrolyte complexes; Cytotoxicity; Osteoblasts; DRUG-DELIVERY; CITRUS PECTIN; STEM-CELLS; CHITOSAN; MATRIX; DIFFERENTIATION; MICROSPHERES; BIOPOLYMERS; OSTEOBLASTS; COMPOSITE;

D O I：

10.1016/j.ijbiomac.2010.10.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

In this work, porous scaffolds obtained from the freeze-drying of pectin/chitosan polyelectrolyte complexes were prepared and characterized by FTIR, SEM and weight loss studies. Additionally, the cytotoxicity of the prepared scaffolds was evaluated in vitro, using human osteoblast cells. The results obtained showed that cells adhered to scaffolds and proliferated. The study also confirmed that the degradation by-products of pectin/chitosan scaffold are noncytotoxic. (C) 2010 Elsevier B.V. All rights reserved.

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页码：112 / 118

页数：7

相关论文

共 44 条

[1]

Development of pectin matrix tablets for colonic delivery of model drug ropivacaine [J].

Ahrabi, SF ;

Madsen, G ;

Dyrstad, K ;

Sande, SA ;

Graffner, C .

EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2000, 10 (01) :43-52

[2]

[Anonymous], 2004, INFRARED SPECTROSCOP, DOI [10.1002/0470011149, DOI 10.1002/0470011149]

[3]

Osteogenic differentiation of dura mater stem cells cultured in vitro on three-dimensional porous scaffolds of poly(ε-caprolactone) fabricated via co-extrusion and gas foaming [J].

Aronin, C. E. Petrie ;

Cooper, J. A., Jr. ;

Sefcik, L. S. ;

Tholpady, S. S. ;

Ogle, R. C. ;

Botchwey, E. A. .

ACTA BIOMATERIALIA, 2008, 4 (05) :1187-1197

[4]

STUDIES ON PECTIN FORMULATIONS FOR COLONIC DRUG-DELIVERY [J].

ASHFORD, M ;

FELL, J ;

ATTWOOD, D ;

SHARMA, H ;

WOODHEAD, P .

JOURNAL OF CONTROLLED RELEASE, 1994, 30 (03) :225-232

[5]

Endocultivation: 3D printed customized porous scaffolds for heterotopic bone induction [J].

Becker, Stephan T. ;

Bolte, Hendrik ;

Krapf, Oliver ;

Seitz, Hermann ;

Douglas, Timothy ;

Sivananthan, Sureshan ;

Wiltfang, Joerg ;

Sherry, Eugene ;

Warnke, Patrick H. .

ORAL ONCOLOGY, 2009, 45 (11) :E181-E188

[6]

Structure and interactions in chitosan hydrogels formed by complexation or aggregation for biomedical applications [J].

Berger, J ;

Reist, M ;

Mayer, JM ;

Felt, O ;

Gurny, R .

EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, 2004, 57 (01) :35-52

[7]

Pectin-based microspheres for colon-specific delivery of vancomycin [J].

Bigucci, Federica ;

Luppi, Barbara ;

Monaco, Libera ;

Cerchiara, Teresa ;

Zecchi, Vittorio .

JOURNAL OF PHARMACY AND PHARMACOLOGY, 2009, 61 (01) :41-46

[8]

Colon-specific drug delivery: Influence of solution reticulation properties upon pectin beads performance [J].

Chambin, O. ;

Dupuis, G. ;

Champion, D. ;

Voilley, A. ;

Pourcelot, Y. .

INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2006, 321 (1-2) :86-93

[9]

FT-IR spectroscopic determination of the degree of esterification of cell wall pectins from stored peaches and correlation to textural changes [J].

Chatjigakis, AK ;

Pappas, C ;

Proxenia, N ;

Kalantzi, O ;

Rodis, P ;

Polissiou, M .

CARBOHYDRATE POLYMERS, 1998, 37 (04) :395-408

[10]

Suppression of endotoxin-induced proinflammatory responses by citrus pectin through blocking LPS signaling pathways [J].

Chen, Chien-Ho ;

Sheu, Ming-Thau ;

Chen, Tzeng-Fu ;

Wang, Ying-Ching ;

Hou, Wen-Chi ;

Liu, Der-Zen ;

Chung, Tsao-Chuen ;

Liang, Yu-Chih .

BIOCHEMICAL PHARMACOLOGY, 2006, 72 (08) :1001-1009

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