Effect of chondroitin sulfate and hyaluronic acid on gene expression in a three-dimensional culture of chondrocytes

被引：55

作者：

Nishimoto, S

Takagi, M

Wakitani, S

Nihira, T

Yoshida, T

机构：

[1] Osaka Univ, Int Ctr Biotechnol, Suita, Osaka 5650871, Japan

[2] Shinshu Univ, Sch Med, Matsumoto, Nagano 3908621, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2005年 / 100卷 / 01期

关键词：

chondrocyte; chondroitin sulfate; hyaluronic acid; RT-PCR;

D O I：

10.1263/jbb.100.123

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effect of glycosaminoglycan addition on a three-dimensional (3D) culture of porcine chondrocyte cells was investigated with a view to use in cartilage regenerative medicine. Chondroitin sulfate C increased the mRNA expression of type 2 collagen, while chondroitin sulfate A did not. Hyaluronic acid of high molecular weight markedly decreased the mRNA expression of both aggrecan and type 2 collagen, although hyaluronic acid of low molecular weight showed no apparent effect.

引用

页码：123 / 126

页数：4

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