Low genetic diversity and significant population structuring in the relict Amentotaxus argotaenia complex (Taxaceae) based on ISSR fingerprinting

被引:67
作者
Ge, XJ
Zhou, XL
Li, ZC
Hsu, TW
Schaal, BA
Chiang, TY
机构
[1] Natl Cheng Kung Univ, Dept Life Sci, Tainan 701, Taiwan
[2] Washington Univ, Dept Biol, St Louis, MO 63130 USA
[3] Taiwan Endem Species Res Inst, Div Bot, Chichi 551, Taiwan
[4] Chinese Acad Sci, S China Bot Garden, Guangzhou 510650, Peoples R China
[5] Sun Yat Sen Univ, State Key Lab Biocontrol, Sch Life Sci, Guangzhou 510275, Peoples R China
基金
中国国家自然科学基金;
关键词
Amentotaxus argotaenia; A; formosana; A.yunnanensis; genetic diversity; ISSR fingerprinting; population structuring; species complex;
D O I
10.1007/s10265-005-0235-1
中图分类号
Q94 [植物学];
学科分类号
071001 [植物学];
摘要
Amentotaxus, a genus of the Taxaceae, represents an ancient lineage that has long existed in Eurasia. All Amentotaxus species experienced frequent population expansion and contraction over periodical glaciations in Tertiary and Quaternary. Among them, Amentotaxus argotaenia complex consists of three morphologically alike species, A. argotaenia, Amentotaxus yunnanensis, and Amentotaxus formosana. This complex is distributed in the subtropical region of mainland China and Taiwan where many Pleistocene refugia have been documented. In this study, genetic diversity and population structuring within and between species were investigated based on the intersimple sequence repeats (ISSR) fingerprinting. Mean genetic diversity within populations was estimated in three ways: (1) the percentage of polymorphic loci out of all loci (P) (2) Nei's unbiased expected heterozygosity (He), and (3) Shannon's index of phenotypic diversity. For a total of 310 individuals of 15 populations sampled from the three species, low levels of ISSR genetic variation within populations were detected, with P =4.66-16.58%, He = 0.01760.0645 and Hpop = 0.0263-0.0939, agreeing with their seriously threatened status. AMOVA analyses revealed that the differences between species only accounted for 27.38% of the total variation, whereas differences among populations and within populations were 57.70 and 14.92%, respectively, indicating substantial isolation between the patch-like populations. A neighbor-joining tree identified a close affinity between A. yunnanensis and A. formosana. Genetic drift due to small population size, plus limited current gene flow, resulted in significant genetic structuring. Low levels of intrapopulational genetic variation and considerable interpopulational divergence were also attributable to demographic bottlenecks during and/or after the Pleistocene glaciations.
引用
收藏
页码:415 / 422
页数:8
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