Liquid chromatographic determination of less polar ginsenosides in processed ginseng

被引：225

作者：

Kwon, SW

Han, SB

Park, IH

Kim, JM

Park, MK

Park, JH ^{[1
]}

机构：

[1] Seoul Natl Univ, Coll Pharm, Res Inst Pharmaceut Sci, Seoul 151742, South Korea

[2] Seoul Clin Labs, Seoul 140230, South Korea

[3] Korean Inst Oriental Med, Kangnam Gu, Seoul 135100, South Korea

来源：

JOURNAL OF CHROMATOGRAPHY A | 2001年 / 921卷 / 02期

关键词：

Panax ginseng; ginsenosides;

D O I：

10.1016/S0021-9673(01)00869-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Reversed-phase LC with an evaporative light scattering detector (ELSD) is used for the determination of less polar ginsenosides in processed ginseng. These ginsenosides include ginsenosides F-4, Rg(3), Rg(5), Rg(6), Rk(1), Rk(3), Rs(3), Rs(4), and Rs(5). The method used a C-18-bonded silica column with a CH3CN/H2O/CH3COOH gradient elution. (20R) and (20S) epimers and geometric isomers at the C-20 position of ginsenosides, which are not generally separated by amino columns, were now clearly separated. (C) 2001 Elsevier Science BN. All rights reserved.

引用

页码：335 / 339

页数：5

共 10 条

[1] INFLUENCE OF VARIOUS PARAMETERS ON THE RESPONSE FACTORS OF THE EVAPORATIVE LIGHT-SCATTERING DETECTOR FOR A NUMBER OF NON-VOLATILE COMPOUNDS [J].