Production of transgenic lily plants by Agrobacterium-mediated transformation

被引：81

作者：

Hoshi, Y

Kondo, M

Mori, S

Adachi, Y

Nakano, M

Kobayashi, H

机构：

[1] Niigata Agr Res Inst, Niigata 9400826, Japan

[2] Niigata Univ, Fac Agr, Niigata 9502181, Japan

来源：

PLANT CELL REPORTS | 2004年 / 22卷 / 06期

关键词：

Agrobacterium tumefaciens; filament-derived callus; Lilium; scratching-treatment; transgenic plant;

D O I：

10.1007/s00299-003-0700-z

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A system for the production of transgenic plants was developed for the Oriental hybrid lily, Lilium cv. Acapulco, by Agrobacterium-mediated genetic transformation. Filament-derived calli were co-cultivated with A. tumefaciens strain EHA101/pIG121Hm, which harbored a binary vector carrying the neomycin phosphotransferase II, hygromycin phosphotransferase, and intron-containing beta-glucuronidase genes in the T-DNA region. Six hygromycin-resistant (Hyg(r)) culture lines were obtained from 200 calli by scratching them with sandpaper prior to inoculation and using NH4NO3-free medium for co-cultivation and a hygromycin-containing regeneration medium for selection. Hyg(r) culture lines regenerated shoots, which developed into plantlets following transfer to a plant growth regulator-free medium. All of these plantlets were verified to be transgenic by GUS histochemical assay and inverse PCR analysis.

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页码：359 / 364

页数：6

相关论文

共 37 条

[1] NUCLEAR-DNA AMOUNTS IN ANGIOSPERMS [J].

BENNETT, MD ;

SMITH, JB .

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1991, 334 (1271) :309-345

[2] MICROPROJECTILE BOMBARDMENT OF PLANT-TISSUES INCREASES TRANSFORMATION FREQUENCY BY AGROBACTERIUM-TUMEFACIENS [J].

BIDNEY, D ;

SCELONGE, C ;

MARTICH, J ;

BURRUS, M ;

SIMS, L ;

HUFFMAN, G .

PLANT MOLECULAR BIOLOGY, 1992, 18 (02) :301-313

[3] Susceptibility of common and tepary beans to Agrobacterium spp strains and improvement of Agrobacterium-mediated transformation using microprojectile bombardment [J].

Brasileiro, ACM ;

Aragao, FJL ;

Rossi, S ;

Dusi, DMA ;

Barros, LMG ;

Rech, EL .

JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE, 1996, 121 (05) :810-815

[4]

Cheng YH, 1996, PLANT CELL REP, V16, P127, DOI 10.1007/BF01890852

[5] Agrobacterium cells as microprojectile coating:: a novel approach to enhance stable transformation rates in strawberry [J].

de Mesa, MC ;

Jiménez-Bermúdez, S ;

Pliego-Alfaro, F ;

Quesada, MA ;

Mercado, JA .

AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY, 2000, 27 (12) :1093-1100

[6] A QUICK METHOD TO ESTIMATE THE T-DNA COPY NUMBER IN TRANSGENIC PLANTS AT AN EARLY STAGE AFTER TRANSFORMATION, USING INVERSE PCR [J].

DOES, MP ;

DEKKER, BMM ;

DEGROOT, MJA ;

OFFRINGA, R .

PLANT MOLECULAR BIOLOGY, 1991, 17 (01) :151-153

[7] Agrobacterium tumefaciens-mediated transformation and transgenic-plant regeneration of onion (Allium cepa L.) [J].

Eady, CC ;

Weld, RJ ;

Lister, CE .

PLANT CELL REPORTS, 2000, 19 (04) :376-381

[8] Gene stability in transgenic aspen (Populus).: I.: Flanking DNA sequences and T-DNA structure [J].

Fladung, M .

MOLECULAR AND GENERAL GENETICS, 1999, 260 (06) :574-581

[9] INTEGRATION OF AGROBACTERIUM-TUMEFACIENS TRANSFER DNA (T-DNA) INVOLVES REARRANGEMENTS OF TARGET PLANT DNA-SEQUENCES [J].

GHEYSEN, G ;

VANMONTAGU, M ;

ZAMBRYSKI, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (17) :6169-6173

[10]

GRAYBURN WS, 1995, PLANT CELL REP, V14, P285, DOI 10.1007/BF00232029

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