Macular pigments lutein and zeaxanthin as blue light filters studied in liposomes

被引:171
作者
Junghans, A
Sies, H
Stahl, W
机构
[1] Univ Dusseldorf, Inst Physiol Chem 1, D-40001 Dusseldorf, Germany
[2] Univ Dusseldorf, Biol Med Forschungszentrum, D-40001 Dusseldorf, Germany
关键词
carotenoids; blue light filter; unilamellar liposomes; lutein; zeaxanthin;
D O I
10.1006/abbi.2001.2411
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Lutein and zeaxanthin are the predominant carotenoids in the human macula lutea. Epidemiological data suggest that an increased intake of a lutein-rich diet correlates with a diminished risk for age-related macular degeneration, a major cause of impaired vision in the elderly. Filtering of blue light has been proposed as a possible mechanism of protection. Here, the blue light filter efficacy of carotenoids was investigated in unilamellar liposomes loaded in the hydrophilic core space with a fluorescent dye, Lucifer yellow, excitable by blue light. Carotenoids were incorporated into the lipophilic membrane. Fluorescence emission in carotenoid-containing liposomes was lower than in carotenoid-free controls when exposed to blue light, indicating a filter effect. Filter efficacy was in the order lutein > zeaxanthin > beta -carotene > lycopene. Some of the difference in blue light filter efficacy of carotenoids is attributable to differences in extinction coefficients, and a major further contribution is suggested to be related to the orientation of the incorporated molecules in the liposomal membrane. (C) 2001 Academic Press.
引用
收藏
页码:160 / 164
页数:5
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