Characterization of oligosaccharides from an antigenic mannan of Saccharomyces cerevisiae

被引：55

作者：

Young, M

Davies, MJ

Bailey, D

Gradwell, MJ

Smestad-Paulsen, B

Wold, JK

Barnes, RMR

Hounsell, EF

机构：

[1] UCL, Dept Biochem & Mol Biol, London WC1E 6BT, England

[2] Natl Inst Med Res, MRC, Biomed NMR Ctr, London NW7 1AA, England

[3] Univ Oslo, Inst Pharm, N-0316 Oslo, Norway

[4] Univ Liverpool, Dept Immunol, Liverpool L69 3BX, Merseyside, England

来源：

GLYCOCONJUGATE JOURNAL | 1998年 / 15卷 / 08期

关键词：

Saccharomyces cerevisiae; oligosaccharide structure; antigenic glycoprotein; mannan; allergens;

D O I：

10.1023/A:1006968117252

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mannans of the yeast Saccharomyces cerevisiae have been implicated as containing the allergens to which bakers and brewers are sensitive and also the antigen recognized by patients with Crohn's disease. A fraction of S. cerevisiae mannan, Sc500, having high affinity for antibodies in Crohn's patients has been characterized by NMR spectroscopy followed by fragmentation using alkaline elimination, partial acid hydrolysis and acetolysis. The released oligosaccharides were separated by gel filtration on a Biogel P4 column and analyzed by fluorescence labeling, HPLC and methylation analysis. The relationship between structure and antigen activity was measured by competitive ELISA, The antigenic activity of the original high molecular weight mannan could be ascribed to terminal Man alpha 1 --> 3Man alpha 1 --> 2 sequences which are rarely found in human glycoproteins but were over-represented in Sc500 compared to other yeast mannans.

引用

页码：815 / 822

页数：8

共 33 条

[1]

ASHTON PR, 1997, IN PRESS J ORG CHEM

[2]

Baldwin CI, 1998, CLIN EXP ALLERGY, V28, P349

[3]

Ballou C, 1976, Adv Microb Physiol, V14, P93, DOI 10.1016/S0065-2911(08)60227-1

[4] SERUM ANTIBODIES REACTIVE WITH SACCHAROMYCES-CEREVISIAE IN INFLAMMATORY BOWEL-DISEASE - IS IGA ANTIBODY A MARKER FOR CROHNS-DISEASE [J].