Screening and mutational improvement of a D-ribose secreting Candida pelliculosa strain

被引:14
作者
DeWulf, P
Soetaert, W
Schwengers, D
Vandamme, EJ
机构
[1] STATE UNIV GHENT, FAC AGR & APPL BIOL SCI, LAB IND MICROBIOL & BIOCATALYSIS, B-9000 GHENT, BELGIUM
[2] PFEIFER & LANGEN, D-41539 DORMAGEN KOLN, GERMANY
来源
JOURNAL OF FERMENTATION AND BIOENGINEERING | 1996年 / 82卷 / 01期
关键词
screening; D-ribose; transketolase; mutation; Candida pelliculosa; D-arabitol;
D O I
10.1016/0922-338X(96)89446-3
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The application of D-ribose in chemistry and pharmacy lately has received much attention. In order to develop an efficient D-ribose production process, a screening strategy was established to isolate naturally occurring D-ribose secreting microorganisms. Of the more than two thousand strains isolated, only four excreted D-ribose in a range from 0.04 to 0.36 g/l, starting from 30 g/l of D-glucose. One strain, identified as Candida pelliculosa, quantitatively co-produced D-arabitol (up to 7 g/l). This strain was subsequently submitted to UV-mutagenesis and a selective nystatin-based enrichment, leading to the isolation of a D-arabitol non-secreting mutant (C. pelliculosa PDW 43 ARA(-)), which synthesized 5 g/l of D-ribose from 30 g/l of D-glucose.
引用
收藏
页码:1 / 7
页数:7
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