Diazoxide effects on hypothalamic and extra-hypothalamic NPY content in Zucker rats

被引：11

作者：

Hensley, IE

Lawler, JE

Alemzadeh, R

Holshouser, SJ

机构：

[1] Univ Tennessee, Med Ctr, Dept Pediat Res, Knoxville, TN 37920 USA

[2] Univ Tennessee, Dept Psychol, Knoxville, TN 37996 USA

来源：

PEPTIDES | 2001年 / 22卷 / 06期

关键词：

neuropeptide Y; insulin; diazoxide; hypothalamus; Zucker rat; radioimmunoassay; micropunch procedure; obesity;

D O I：

10.1016/S0196-9781(01)00415-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To determine if the anorectic effects of the insulin antagonist diazoxide (DZ) are mediated by reduced central neuropeptide Y (NPY), female Zucker rats, given DZ(150 mg/kg/day) or placebo for about four weeks, were sacrificed following overnight fasting or free feeding. Several hypothalamic and extra-hypothalamic nuclei were extracted for NPY content. DZ reduced weight gain in obese rats and lowered glucose of lean and obese rats without affecting insulin. Contrary to the hypothesis, DZ increased NPY in hypothalamic nuclei of free fed lean and obese rats. DZ elevated hypothalamic NPY levels in fasted obese rats and had more diverse effects in extra-hypothalamic nuclei of lean rats. (C) 2001 Elsevier Science Inc. All rights reserved.

引用

页码：899 / 908

页数：10

共 62 条

[1] NEUROPEPTIDE-Y IN THE ARCUATE NUCLEUS IS MODULATED BY ALTERATIONS IN GLUCOSE-UTILIZATION [J].