RNA sequencing: advances, challenges and opportunities

被引：1402

作者：

Ozsolak, Fatih ^{[1
]}

Milos, Patrice M. ^{[1
]}

机构：

[1] Helicos BioSci Corp, Cambridge, MA 02139 USA

来源：

NATURE REVIEWS GENETICS | 2011年 / 12卷 / 02期

关键词：

GENOME-WIDE ANALYSIS; GENE-EXPRESSION; AMPLIFICATION-FREE; CELL TRANSCRIPTOME; REGULATORY ROLES; SMALL SAMPLES; TINY RNAS; REVEALS; SEQ; IDENTIFICATION;

D O I：

10.1038/nrg2934

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

In the few years since its initial application, massively parallel cDNA sequencing, or RNA-seq, has allowed many advances in the characterization and quantification of transcriptomes. Recently, several developments in RNA-seq methods have provided an even more complete characterization of RNA transcripts. These developments include improvements in transcription start site mapping, strand-specific measurements, gene fusion detection, small RNA characterization and detection of alternative splicing events. Ongoing developments promise further advances in the application of RNA-seq, particularly direct RNA sequencing and approaches that allow RNA quantification from very small amounts of cellular materials.

引用

页码：87 / 98

页数：12

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