Improvement of RT-PCR detection of chronic bee paralysis virus (CBPV) required by the description of genomic variability in French CBPV isolates

被引：35

作者：

Blanchard, Philippe ^{[1
]}

Olivier, Violaine ^{[1
]}

Iscache, Anne-Laure ^{[1
]}

Celle, Olivier ^{[1
]}

Schurr, Frank ^{[1
]}

Lallemand, Perrine ^{[1
]}

Ribiere, Magali ^{[1
]}

机构：

[1] Unite Pathol Abeille, AFSSA Sophia Antipolis, FR-06902 Sophia Antipolis, France

来源：

JOURNAL OF INVERTEBRATE PATHOLOGY | 2008年 / 97卷 / 02期

关键词：

Chronic bee paralysis virus (CBPV); RT-PCR diagnosis; genomic variability; Apis mellifera; APIS-MELLIFERA; PHYLOGENETIC ANALYSIS; INFECTION;

D O I：

10.1016/j.jip.2007.07.003

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

A new RT-PCR test has been developed to diagnose Chronic bee paralysis virus (CBPV) that is able to detect genetically variable viral isolates. In fact, up to 8.7% divergence between partial nucleotide sequences from viral isolates from French honey bees was highlighted in a preliminary variability study. The previously-described RT-PCR was unable to detect all these viral isolates and RT-PCR diagnosis needed improvement. The new RT-PCR test can detect up to 40% more CBPV isolates. (C) 2007 Elsevier Inc. All rights reserved.

引用

页码：182 / 185

页数：4

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