Compartmental oxidation of thiol-disulphide redox couples during epidermal growth factor signalling

被引：133

作者：

Halvey, PJ

Watson, WH

Hansen, JM

Go, YM

Samali, A

Jones, DP

机构：

[1] Emory Univ, Sch Med, Div Pulm Allergy & Crit Care Med, Dept Med, Atlanta, GA 30322 USA

[2] Natl Univ Galway, Dept Biochem, Galway, Ireland

[3] Natl Univ Galway, Natl Ctr Biomed Engn Sci, Galway, Ireland

[4] Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Dept Environm Hlth Sci, Baltimore, MD 21205 USA

来源：

BIOCHEMICAL JOURNAL | 2005年 / 386卷

关键词：

epidermal growth factor (EGF); glutathione; redox signalling; thioredoxin-1; thioredoxin-2;

D O I：

10.1042/BJ20041829

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Exogenously added ROS (reactive oxygen species) cause generalized oxidation of cellular components, whereas endogenously generated ROS induced by physiological stimuli activate discrete signal transduction pathways. Compartmentation is an important aspect of such pathways, but little is known about its role in redox signalling. We measured the redox states of cytosolic and nuclear Trx1 (thioredoxin-1) and mitochondrial Trx2 (thioredoxin-2) using redox Western blot methodologies during endogenous ROS production induced by EGF (epidermal growth factor) signalling. The glutathione redox state was measured by HPLC. Results showed that only cytosolic Trx1 undergoes significant oxidation. Thus EGF signalling involves subcellular compartmental oxidation of Trx1 in the absence of a generalized cellular oxidation.

引用

页码：215 / 219

页数：5

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