The effect of PAMAM G6 dendrimers on the structure of lipid vesicles

被引:28
作者
Akesson, Anna [1 ,2 ]
Bendtsen, Kristian Moss [1 ,2 ]
Beherens, Manja A. [3 ,4 ]
Pedersen, Jan Skov [3 ,4 ]
Alfredsson, Viveka [5 ]
Gomez, Marite Cardenas [1 ,2 ]
机构
[1] Univ Copenhagen, Nanosci Ctr, DK-2100 Copenhagen E, Denmark
[2] Univ Copenhagen, Inst Chem, DK-2100 Copenhagen E, Denmark
[3] Univ Aarhus, Dept Chem, DK-8000 Aarhus C, Denmark
[4] Univ Aarhus, iNANO Interdisciplinary Nanosci Ctr, DK-8000 Aarhus C, Denmark
[5] Lund Univ, Ctr Chem & Chem Engn, SE-22100 Lund, Sweden
关键词
POLY(AMIDOAMINE) DENDRIMERS; BILAYER DISRUPTION; PHASE; SURFACTANT; MEMBRANES; POLYMERS;
D O I
10.1039/c0cp00172d
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Dendrimers are polymers with unique properties that make them promising in a variety of applications such as potential drug and gene delivery systems. PAMAM dendrimers, in particular, have been widely investigated and are efficiently translocated into the cell. The mechanism of translocation, however, is still unknown. Recently it was proposed that PAMAM dendrimers are able to open holes in lipid bilayers by stealing lipid from the bilayer and forming "dendrisomes''. The present work intends to contribute in the clarification of this question: why are dendrimers able to translocate into the cell? We create simple models for cell membranes by using small lipid vesicles that present a single lipid phase at physiologically relevant conditions. We then follow the effect that dendrimers have on the structure of the vesicles by using a combination of various techniques: dynamic light scattering, cryo-TEM and small angle X-ray scattering. We discuss our results with respect to the previous findings and reflect on their possible implications for real translocation in living cells.
引用
收藏
页码:12267 / 12272
页数:6
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