Osmotic compaction of supercoiled DNA into a bacterial nucleoid

被引：122

作者：

Odijk, T ^{[1
]}

机构：

[1] Delft Univ Technol, Fac Chem Engn & Mat Sci, NL-2600 GA Delft, Netherlands

来源：

BIOPHYSICAL CHEMISTRY | 1998年 / 73卷 / 1-2期

关键词：

supercoiled DNA; bacterial cell; phase separation; liquid crystal; nucleoid; proteins;

D O I：

10.1016/S0301-4622(98)00115-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A theory is presented of the phase separation of supercoiled DNA into a nucleoid in a bacterial cell. The suspension consists of DNA interacting with globular proteins in excess salt. A cross virial between DNA and a protein is computed as well as the DNA self-energy arising from excluded volume. The cellular parameters of Escherichia coli would appear to be compatible with the thermodynamic equilibrium derived theoretically. The state of superhelical DNA in the nucleoid could be liquid crystalline and rippled. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：23 / 29

页数：7

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