Retroviral expression screening of oncogenes in natural killer cell leukemia

被引：10

作者：

Choi, YL

Moriuchi, R

Osawa, M

Iwama, A

Makishima, H

Wada, T

Kisanuki, H

Kaneda, R

Ota, J

Koinuma, K

Ishikawa, M

Takada, S

Yamashita, Y

Oshimi, K

Mano, H

机构：

[1] Jichi Med Sch, Div Funct Genom, Kawachi, Tochigi 3290498, Japan

[2] Juntendo Univ, Sch Med, Dept Med, Div Hematol, Tokyo 113, Japan

[3] Nagasaki Univ, Grad Sch Med, Dept Mol Microbiol & Immunol, Nagasaki 852, Japan

[4] Univ Tokyo, Inst Med Sci, Ctr Med Expt, Tokyo, Japan

[5] Shinshu Univ, Sch Med, Dept Internal Med 2, Nagano, Japan

来源：

LEUKEMIA RESEARCH | 2005年 / 29卷 / 08期

关键词：

aggressive NK cell leukemia; cDNA expression library; retrovirus; KRAS2; oncogene;

D O I：

10.1016/j.leukres.2005.02.002

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Aggressive natural killer cell leukemia (ANKL) is an intractable malignancy that is characterized by the outgrowth of NK cells. To identify transforming genes in ANKL, we constructed a retroviral cDNA expression library from an ANKL cell line KHYG-1. Infection of 3T3 cells with recombinant retroviruses yielded 33 transformed foci. Nucleotide sequencing of the DNA inserts recovered from these foci revealed that 31 of them encoded KRAS2 with a glycine-to-alanine mutation at codon 12. Mutation-specific PCR analysis indicated that the KRAS mutation was present only in KHYG-1 cells, not in another ANKL cell line or in clinical specimens (n = 8). (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：943 / 949

页数：7

共 23 条

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