Morphological study of adult male worms of Schistosoma mansoni Sambon, 1907 by confocal laser scanning microscopy

被引:39
作者
Machado-Silva, JR
Pelajo-Machado, M
Lenzi, HL
Gomes, DC
机构
[1] UERJ, Fac Ciencias Med, Dept Patol & Lab, BR-20551031 Rio De Janeiro, RJ, Brazil
[2] Inst Oswaldo Cruz, Lab Helmintos Parasitos Vertebrados, Dept Helmintol, BR-21045900 Rio De Janeiro, RJ, Brazil
来源
MEMORIAS DO INSTITUTO OSWALDO CRUZ | 1998年 / 93卷
关键词
Schistosoma mansoni; adult male; morphology; confocal laser scanning microscopy; reproductive system;
D O I
10.1590/S0074-02761998000700060
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Aiming to detail data obtained through brightfield microscopy (BM) on reproductive, excretory and digestive system, specimens of Schistosoma mansoni eight weeks old, were recovered from SW mice, stained with Langeron's carmine and analyzed under a confocal laser scanning microscope CLSM 410 (Carl Zeiss). The reproductive system presented a single and lobate testis, with intercommunications between the lobes without efferent duct. Supernumerary testicular lobe was amorphous and isolated from the normal ones. Collecting tubules (excretory ducts), followed by the excretory bladder opening to the external media through the excretory pore, were observed at the posterior extremity of the body. In the digestive tract, a cecal swelling was noted at the junction that originates the single cecum. It was concluded that through confocal laser scanning microscopy, new interpretations of morphological structures of S. mansoni worms could be achieved, modifying adopted and current descriptions. The gonad consists of a single lobed testis, similar to that observed in some trematode species. Moreover the same specimens can be observed either by BM or CLSM, considering that the latter causes only focal and limited damage in tissue structures.
引用
收藏
页码:303 / 307
页数:5
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