Requirement for the SLP-76 adaptor CADS in T cell development

被引：124

作者：

Yoder, J

Pham, C

Iizuka, YM

Kanagawa, O

Liu, SK

McGlade, J

Cheng, AM ^{[1
]}

机构：

[1] Washington Univ, Sch Med, Ctr Immunol, St Louis, MO 63110 USA

[2] Washington Univ, Sch Med, Med Sci Training Program, St Louis, MO 63110 USA

[3] Washington Univ, Sch Med, Dept Med, Div Rheumatol, St Louis, MO 63110 USA

[4] Washington Univ, Sch Med, Dept Pathol & Immunol, St Louis, MO 63110 USA

[5] Univ Toronto, Hosp Sick Children, Toronto, ON M5G 1X8, Canada

来源：

SCIENCE | 2001年 / 291卷 / 5510期

关键词：

D O I：

10.1126/science.1057176

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

GADS is an adaptor protein implicated in CD3 signaling because of its ability to link SLP-76 to LAT. A GADS-deficient mouse was generated by gene targeting, and the function of GADS in T cell development and activation was examined. GADS(-) CD4(-)CD8(-) thymocytes exhibited a severe block in proliferation but still differentiated into mature T cells. GADS(-) thymocytes failed to respond to CD3 cross-linking in vivo and were-impaired in positive and negative selection. Immunoprecipitation experiments revealed that the association between SLP-76 and LAT was uncoupled in GADS(-) thymocytes. These observations indicate that CADS is a critical adaptor for CD3 signaling.

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页码：1987 / 1991

页数：5

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