Use of PCR to diagnose Toxoplasma gondii chorioretinitis in eyes with severe vitritis

被引：10

作者：

Okhravi, N

Jones, CD

Carroll, N

Adamson, P

Luthert, P

Lightman, S

机构：

[1] Moorfields Eye Hosp, Dept Clin Ophthalmol, Inst Ophthalmol, London EC1V 2PD, England

[2] Moorfields Eye Hosp, Dept Pathol, Inst Ophthalmol, London EC1V 2PD, England

来源：

CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY | 2005年 / 33卷 / 02期

关键词：

paraffin; PCR; Toxoplasma gondii; vitreous; vitritis;

D O I：

10.1111/j.1442-9071.2005.00992.x

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Two cases are reported of intraocular inflammation in which severe vitritis hampered the fundal view, making an accurate clinical diagnosis impossible, and vitreous analysis using conventional techniques was unhelpful. PCR for Toxoplasma gondii was positive in both cases and provided the only way of confirming the diagnosis. Other ocular samples also underwent PCR for Toxoplasma DNA and the specificity of this approach is demonstrated.

引用

收藏

页码：184 / 187

页数：4

相关论文

共 11 条

[1] Reactivations of ocular toxoplasmosis after cataract extraction [J].

Bosch-Driessen, LH ;

Plaisier, MB ;

Stilma, JS ;

Van der Lelij, A ;

Rothova, A .

OPHTHALMOLOGY, 2002, 109 (01) :41-45

[2] Polymerase chain reaction for the detection of Toxoplasma gondii within aqueous humor of experimentally-inoculated cats [J].

Burney, DP ;

Chavkin, MJ ;

Dow, SW ;

Potter, TA ;

Lappin, MR .

VETERINARY PARASITOLOGY, 1998, 79 (03) :181-186

[3] Diagnosis of toxoplasmic retinochoroiditis with atypical clinical features [J].

Fardeau, C ;

Romand, S ;

Rao, NA ;

Cassoux, N ;

Bettembourg, O ;

Thulliez, P ;

Lehoang, P .

AMERICAN JOURNAL OF OPHTHALMOLOGY, 2002, 134 (02) :196-203

[4]

Jones CD, 2000, INVEST OPHTH VIS SCI, V41, P634

[5] AQUEOUS-HUMOR ANALYSIS AS A DIAGNOSTIC-TOOL IN TOXOPLASMA UVEITIS [J].

KIJLSTRA, A ;

LUYENDIJK, L ;

BAARSMA, GS ;

ROTHOVA, A ;

SCHWEITZER, CMC ;

TIMMERMAN, Z ;

DEVRIES, J ;

BREEBAART, AC .

INTERNATIONAL OPHTHALMOLOGY, 1989, 13 (06) :383-386

[6] Ocular toxoplasmosis after the fifth decade [J].

Labalette, P ;

Delhaes, L ;

Margaron, F ;

Fortier, B ;

Rouland, JF .

AMERICAN JOURNAL OF OPHTHALMOLOGY, 2002, 133 (04) :506-515

[7] USE OF THE POLYMERASE CHAIN-REACTION IN THE DIAGNOSIS OF ACQUIRED OCULAR TOXOPLASMOSIS IN AN IMMUNOCOMPETENT ADULT [J].

MANNERS, RM ;

OCONNELL, S ;

GUY, EC ;

JOYNSON, DHM ;

CANNING, CR ;

ETCHELLS, DE .

BRITISH JOURNAL OF OPHTHALMOLOGY, 1994, 78 (07) :583-584

[8] Use of the polymerase chain reaction for diagnosis of ocular toxoplasmosis [J].

Montoya, JG ;

Parmley, S ;

Liesenfeld, O ;

Jaffe, GJ ;

Remington, JS .

OPHTHALMOLOGY, 1999, 106 (08) :1554-1563

[9]

Theaudin M, 2003, J FR OPHTALMOL, V26, P921

[10] Spindle cells isolated from Kaposi's sarcoma-like lesions of BKV/tat-transgenic mice co-express markers of different cell types [J].

Cavallaro, U ;

Gasparini, G ;

Soria, MR ;

Maier, JAM .

AIDS, 1996, 10 (11) :1211-1219