Novel polymorphic microsatellites for the red-legged partridge (Alectoris rufa) and cross-species amplification in Alectoris graeca

被引：20

作者：

Gonzalez, EG ^{[1
]}

Castilla, AM ^{[1
]}

Zardoya, R ^{[1
]}

机构：

[1] CSIC, Museo Nacl Ciencias Nat, Dept Biodivers & Biol Evolut, E-28006 Madrid, Spain

来源：

MOLECULAR ECOLOGY NOTES | 2005年 / 5卷 / 02期

关键词：

game bird; hybridization; microsatellite; red-legged partridge; rock partridge;

D O I：

10.1111/j.1471-8286.2005.00960.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The red-legged partridge, Alectoris rufa, is an endemic species of the southwestern Mediterranean, and the most popular game bird in the Iberian Peninsula. A total of 27 microsatellite loci was isolated from an enriched genomic library of A. rufa. Six perfect GT microsatellites were characterized and optimized in 45 individuals of A. rufa. All loci revealed high levels of polymorphism with a number of alleles that ranged from three to 13. Observed heterozygosity ranged from 0.2 to 0.6. Cross-species amplification showed that all loci were also polymorphic in rock partridge, Alectoris graeca. The new markers will be useful in determining hybridization between both species of Alectoris.

引用

页码：449 / 451

页数：3

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