Osteogenic differentiation of adipose-derived mesenchymal stem cells into Polycaprolactone (PCL) scaffold

被引：29

作者：

Caetano, Guilherme Ferreira ^{[1
]}

Bartolo, Paulo Jorge ^{[2
]}

Domingos, Marco ^{[2
]}

Oliveira, Carolina Caliari ^{[3
]}

Leite, Marcel Nani ^{[1
]}

Cipriani Frade, Marco Andrey ^{[1
]}

机构：

[1] Univ Sao Paulo, Dept Internal Med, Ribeirao Preto Sch Med, 3900 Brandeirantes Av, BR-14049900 Ribeirao Preto, Brazil

[2] Univ Manchester, Manchester Biomfg Ctr, Manchester M1 7DN, Lancs, England

[3] Univ Sao Paulo, Sch Med Ribeirao Preto, Basic & Appl Immunol Program, BR-14049900 Ribeirao Preto, Brazil

来源：

4TH INTERNATIONAL CONFERENCE ON TISSUE ENGINEERING, ICTE2015, AN ECCOMAS THEMATIC CONFERENCE | 2015年 / 110卷

关键词：

Scaffolds; Additive fabrication processes; Mesenchymal stem cells; Osteogenesis; Polycaprolactone; UMBILICAL-CORD BLOOD; HUMAN BONE-MARROW; IN-VITRO; TISSUE;

D O I：

10.1016/j.proeng.2015.07.010

中图分类号：

Q813 [细胞工程];

学科分类号：

100113 [医学细胞生物学];

摘要：

The objective of this study was to evaluate the osteogenic differentiation of adipose-derived mesenchymal stem cells (ADSCs) into polycaprolactone scaffold (PCL). After ADSC characterization, cells were seeded onto the scaffolds and incubated with basal or osteogenic differentiating medium to MTT assay and osteogenesis evaluation. MTT cell viability assay showed that cell-seeded scaffolds cultured in both mediums presented formazan cristals formed as a result of the presence of viable cells. The osteogenic assay showed that cell-seeded PCL scaffolds cultured in osteogenic medium presented significantly greater amount of staining dye when compared to basal medium and scaffold without cells. The PCL scaffold have great potential to be used associated to ADSCs in Tissue Engineering to bone tissue regeneration. (C) 2015 Published by Elsevier Ltd.

引用

页码：59 / 66

页数：8

共 24 条

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