Adhesion and proliferation of human osteoblastic cells seeded on injectable hydroxyapatite microspheres

In the present work, the interaction of human osteoblast-like MG63 cells with novel hydroxyapatite (HAp) microspheres was investigated. Cells were seeded on the microspheres and incubated for up to 7 days. The cell-material constructs were visualised by scanning electron microscopy and confocal laser scanning microscopy, and the rate of cell proliferation was estimated using the Neutral Red assay. The results showed that osteoblastic cells were able to adhere and proliferate on the HAp microspheres, which are intended to be used as injectable support-materials for bone regeneration.