Bacterial diversity in the bacterioneuston (sea surface microlayer): the bacterioneuston through the looking glass

被引:89
作者
Franklin, MP
McDonald, IR
Bourne, DG
Owens, NJP
Upstill-Goddard, RC
Murrell, JC [1 ]
机构
[1] Univ Warwick, Dept Biol Sci, Coventry CV4 7AL, W Midlands, England
[2] Newcastle Univ, Sch Marine Sci & Technol, Ocean Res Grp, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England
[3] Plymouth Marine Lab, Plymouth, Devon, England
关键词
D O I
10.1111/j.1462-2920.2004.00736.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The bacterioneuston is defined as the community of bacteria present within the neuston or sea surface microlayer. Bacteria within this layer were sampled using a membrane filter technique and bacterial diversity was compared with that in the underlying pelagic coastal seawater using molecular ecological techniques. 16S rRNA gene libraries of approximate to 500 clones were constructed from both bacterioneuston and the pelagic water samples and representative clones from each library were sequenced for comparison of bacterial diversity. The bacterioneuston was found to have a significantly lower bacterial diversity than the pelagic seawater, with only nine clone types (ecotaxa) as opposed to 46 ecotaxa in the pelagic seawater library. Surprisingly, the bacterioneuston clone library was dominated by 16S rRNA gene sequences affiliated to two groups of organisms, Vibrio spp. which accounted for over 68% of clones and Pseudoalteromonas spp. accounting for 21% of the library. The dominance of these two 16S rRNA gene sequence types within the bacterioneuston clone library was confirmed in a subsequent gene probing experiment. 16S rRNA gene probes specific for these groups of bacteria were designed and used to probe new libraries of 1000 clones from both the bacterioneuston and pelagic seawater DNA samples. This revealed that 57% of clones from the bacterioneuston library hybridized to a Vibrio sp.-specific 16S rRNA gene probe and 32% hybridized to a Pseudoalteromonas sp.-specific 16S rRNA gene probe. In contrast, the pelagic seawater library resulted in only 13% and 8% of 16S rRNA gene clones hybridizing to the Vibrio sp. and Pseudoalteromonas sp. probes respectively. Results from this study suggest that the bacterioneuston contains a distinct population of bacteria and warrants further detailed study at the molecular level.
引用
收藏
页码:723 / 736
页数:14
相关论文
共 58 条
[1]   Comparison of samplers for the biological characterization of the sea surface microlayer [J].
Agogué, H ;
Casamayor, EO ;
Joux, F ;
Obernosterer, I ;
Dupuy, C ;
Lantoine, F ;
Catala, P ;
Weinbauer, MG ;
Reinthaler, T ;
Herndl, GJ ;
Lebaron, P .
LIMNOLOGY AND OCEANOGRAPHY-METHODS, 2004, 2 :213-225
[2]   BASIC LOCAL ALIGNMENT SEARCH TOOL [J].
ALTSCHUL, SF ;
GISH, W ;
MILLER, W ;
MYERS, EW ;
LIPMAN, DJ .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410
[3]   Are readily culturable bacteria in coastal North Sea waters suppressed by selective grazing mortality? [J].
Beardsley, C ;
Pernthaler, J ;
Wosniok, W ;
Amann, R .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (05) :2624-2630
[4]   SURFACE CONCENTRATION OF MARINE BACTERIA [J].
BEZDEK, HF ;
CARLUCCI, AF .
LIMNOLOGY AND OCEANOGRAPHY, 1972, 17 (04) :566-&
[5]   Diversity and association of psychrophilic bacteria in Antarctic sea ice [J].
Bowman, JP ;
McCammon, SA ;
Brown, MV ;
Nichols, DS ;
McMeekin, TA .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (08) :3068-3078
[6]   PHYLOGENETIC ANALYSIS OF A NATURAL MARINE BACTERIOPLANKTON POPULATION BY RIBOSOMAL-RNA GENE CLONING AND SEQUENCING [J].
BRITSCHGI, TB ;
GIOVANNONI, SJ .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (06) :1707-1713
[7]  
CARTY C, 1975, Journal of the Washington Academy of Sciences, V65, P148
[8]   The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy [J].
Cole, JR ;
Chai, B ;
Marsh, TL ;
Farris, RJ ;
Wang, Q ;
Kulam, SA ;
Chandra, S ;
McGarrell, DM ;
Schmidt, TM ;
Garrity, GM ;
Tiedje, JM .
NUCLEIC ACIDS RESEARCH, 2003, 31 (01) :442-443
[9]   High-throughput methods for culturing microorganisms in very-low-nutrient media yield diverse new marine isolates [J].
Connon, SA ;
Giovannoni, SJ .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (08) :3878-3885
[10]   INFLUENCE OF THE SURFACE MICROLAYER ON THE FLUX OF NONCONSERVATIVE TRACE GASES (CO, H-2, CH4, N2O) ACROSS THE OCEAN-ATMOSPHERE INTERFACE [J].
CONRAD, R ;
SEILER, W .
JOURNAL OF ATMOSPHERIC CHEMISTRY, 1988, 6 (1-2) :83-94