Bone morphogenetic protein (BMP)1-3 enhances bone repair

被引：69

作者：

Grgurevic, Lovorka ^{[1
]}

Macek, Boris ^{[2
]}

Mercep, Mladen ^{[3
]}

Jelic, Mislav ^{[1
]}

Smoljanovic, Tomislav ^{[1
]}

Erjavec, Igor ^{[1
]}

Dumic-Cule, Ivo ^{[1
]}

Prgomet, Stefan ^{[1
]}

Durdevic, Dragan ^{[4
]}

Vnuk, Drazen ^{[5
]}

Lipar, Marija ^{[5
]}

Stejskal, Marko ^{[5
]}

Kufner, Vera ^{[1
]}

Brkljacic, Jelena ^{[1
]}

Maticic, Drazen ^{[5
]}

Vukicevic, Slobodan ^{[1
]}

机构：

[1] Univ Zagreb, Lab Mineralized Tissues, Ctr Translat & Clin Res & Orthopaed Surg, Zagreb 10000, Croatia

[2] Univ Tubingen, Proteome Ctr, Interdept Inst Cell Biol, Tubingen, Germany

[3] Res Ctr Zagreb Ltd, GlaxoSmithKline, Zagreb, Croatia

[4] Clin Traumatol, Zagreb 10000, Croatia

[5] Univ Zagreb, Clin Surg Orthopaed & Ophthalmol, Fac Vet Med, Zagreb 10000, Croatia

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2011年 / 408卷 / 01期

关键词：

BMP1-3; Bone fracture; ECM; BMP7; Differentiation; EXPRESSION; MATRIX;

D O I：

10.1016/j.bbrc.2011.03.109

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Members of the astacin family of metalloproteinases such as human bone morphogenetic protein 1 (BMP-1) regulate morphogenesis by processing precursors to mature functional extracellular matrix (ECM) proteins and several growth factors including TGF beta, BMP2, BMP4 and GFD8. We have recently discovered that BMP1-3 isoform of the Bmp-1 gene circulates in the human plasma and is significantly increased in patients with acute bone fracture. We hypothesized that circulating BMP1-3 might have an important role in bone repair and serve as a novel bone biomarker. When administered systemically to rats with a long bone fracture and locally to rabbits with a critical size defect of the ulna, recombinant human BMP1-3 enhanced bone healing. In contrast, neutralization of the endogenous BMP1-3 by a specific polyclonal antibody delayed the bone union. In vitro BMP1-3 increased the expression of collagen type land osteocalcin in MC3T3-E-1 osteoblast like cells, and enhanced the formation of mineralized bone nodules from bone marrow mesenchymal stem cells. We suggest that BMP1-3 is a novel systemic regulator of bone repair. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：26 / +

页数：7

共 28 条

[1] Bone morphogenetic protein 1 is an extracellular processing enzyme of the laminin 5 γ2 chain [J].