A cell for producing xenon-derivative crystals for cryocrystallographic analysis

被引:19
作者
Djinovic-Carugo, K [1 ]
Everitt, P [1 ]
Tucker, PA [1 ]
机构
[1] European Mol Biol Lab, Struct Biol Programme, D-69012 Heidelberg, Germany
来源
JOURNAL OF APPLIED CRYSTALLOGRAPHY | 1998年 / 31卷 / 31期
关键词
D O I
10.1107/S0021889898003513
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A simple pressure cell is described that may be used to bind xenon to protein crystals freely suspended in thin films which may then be flash-cooled, thus immobilizing the bound xenon. As an example, the use of the cell in the structure determination of perchloric-acid-soluble protein, by the multiple isomorphous replacement method, is described.
引用
收藏
页码:812 / 814
页数:3
相关论文
共 8 条
  • [1] [Anonymous], 1995, INT TABLES CRYSTALLO
  • [2] A removable arc for mounting and recovering flash-cooled crystals
    Engel, C
    Wierenga, R
    Tucker, PA
    [J]. JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1996, 29 : 208 - 210
  • [3] FUREY W, 1990, AM CRYST ASS M NEW O
  • [4] Gas access to the active site of Ni-Fe hydrogenases probed by X-ray crystallography and molecular dynamics
    Montet, Y
    Amara, P
    Volbeda, A
    Vernede, X
    Hatchikian, EC
    Field, MJ
    Frey, M
    FontecillaCamps, JC
    [J]. NATURE STRUCTURAL BIOLOGY, 1997, 4 (07) : 523 - 526
  • [5] OKA T, 1995, J BIOL CHEM, V270, P30060, DOI 10.1074/jbc.270.50.30060
  • [6] Processing of X-ray diffraction data collected in oscillation mode
    Otwinowski, Z
    Minor, W
    [J]. MACROMOLECULAR CRYSTALLOGRAPHY, PT A, 1997, 276 : 307 - 326
  • [7] Freeze-trapping isomorphous xenon derivatives of protein crystals
    Sauer, O
    Schmidt, A
    Kratky, C
    [J]. JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1997, 30 (30) : 476 - 486