Mechanical Deformation and Glycosaminoglycan Content Changes in a Rabbit Annular Puncture Disc Degeneration Model

被引:48
作者
Chan, Deva D. [1 ]
Khan, Safdar N. [2 ]
Ye, Xiaojing [2 ]
Curtiss, Shane B. [2 ]
Gupta, Munish C. [2 ]
Klineberg, Eric O. [2 ]
Neu, Corey P. [1 ]
机构
[1] Purdue Univ, Weldon Sch Biomed Engn, W Lafayette, IN 47907 USA
[2] Univ Calif Davis, Med Ctr, Dept Orthopaed Surg, Lawrence J Ellison Musculoskeletal Res Ctr, Sacramento, CA 95817 USA
关键词
rabbit disc degeneration; displacement-encoded MRI; dGEMRIC; cartilage elastography; quantitative MRI; HUMAN INTERVERTEBRAL DISC; NUCLEUS PULPOSUS; ARTICULAR-CARTILAGE; IN-VIVO; LUMBAR SPINE; ANIMAL-MODEL; MRI; DISPLACEMENT; QUANTIFICATION; COMPRESSION;
D O I
10.1097/BRS.0b013e3181f8be52
中图分类号
R74 [神经病学与精神病学];
学科分类号
100204 [神经病学];
摘要
Study Design. Evaluation of degenerated intervertebral discs from a rabbit annular puncture model by using specialized magnetic resonance imaging (MRI) techniques, including displacement encoding with stimulated echoes and a fast-spin echo (DENSE-FSE) acquisition and delayed gadolinium-enhanced MRI of cartilage (dGEMRIC). Objective. To evaluate a rabbit disc degeneration model by using various MRI techniques. To determine the displacements and strains, spin-lattice relaxation time (T(1)), and glycosaminoglycan (GAG) distribution of degenerated discs as compared to normal and adjacent level discs. Summary of Background Data. Annular puncture of the intervertebral disc produces disc degeneration in rabbits. DENSE-FSE has been previously demonstrated in articular cartilage for the measurement of soft tissue displacements and strains. MRI also can measure the T(1) of tissue, and dGEMRIC can quantify GAG concentration in cartilage. Methods. In eight New Zealand white rabbits, the annulus fibrosis of a lumbar disc was punctured. After 4 weeks, the punctured and cranially adjacent motion segments were isolated for MRI and histology. MRI was used to estimate the disc volume and map T(1). DENSE-FSE was used to determine displacements for the estimation of strains. dGEMRIC was then used to determine GAG distributions. Results. Histology and standard MRI indicated degeneration in punctured discs. Disc volume increased significantly at 4 weeks after the puncture. Displacement of the nucleus pulposus was distinct from that of the annulus fibrosis in most untreated discs but not in punctured discs. T(1) was significantly higher and GAG concentration significantly lower in punctured discs compared with untreated adjacent level discs. Conclusion. Noninvasive and quantitative MRI techniques can be used to evaluate the mechanical and biochemical changes that occur with animal models of disc degeneration. DENSE-FSE, dGEMRIC, and similar techniques have potential for evaluating the progression of disc degeneration and the efficacy of treatments.
引用
收藏
页码:1438 / 1445
页数:8
相关论文
共 44 条
[1]
'Stress' distributions inside intervertebral discs - The effects of age and degeneration [J].
Adams, MA ;
McNally, DS ;
Dolan, P .
JOURNAL OF BONE AND JOINT SURGERY-BRITISH VOLUME, 1996, 78B (06) :965-972
[2]
Proteoglycan-induced changes in T1ρ-relaxation of articular cartilage at 4T [J].
Akella, SVS ;
Regatte, RR ;
Gougoutas, AJ ;
Borthakur, A ;
Shapiro, EM ;
Kneeland, JB ;
Leigh, JS ;
Reddy, R .
MAGNETIC RESONANCE IN MEDICINE, 2001, 46 (03) :419-423
[3]
Summary statement - Emerging techniques for treatment of degenerative lumbar disc disease [J].
An, H ;
Boden, SD ;
Kang, J ;
Sandhu, HS ;
Abdu, W ;
Weinstein, J .
SPINE, 2003, 28 (15) :S24-S25
[4]
The human lumbar intervertebral disc - Evidence for changes in the biosynthesis and denaturation of the extracellular matrix with growth, maturation, ageing, and degeneration [J].
Antoniou, J ;
Steffen, T ;
Nelson, F ;
Winterbottom, N ;
Hollander, AP ;
Poole, RA ;
Aebi, M ;
Alini, M .
JOURNAL OF CLINICAL INVESTIGATION, 1996, 98 (04) :996-1003
[5]
Quantitative magnetic resonance imaging of enzymatically induced degradation of the nucleus pulposus of intervertebral discs [J].
Antoniou, John ;
Mwale, Fackson ;
Demers, Caroline N. ;
Beaudoin, Gilles ;
Goswami, Tapas ;
Aebi, Max ;
Alini, Mauro .
SPINE, 2006, 31 (14) :1547-1554
[6]
Glycosaminoglycan in articular cartilage: In vivo assessment with delayed Gd(DTPA)(2-)-enhanced MR imaging [J].
Bashir, A ;
Gray, ML ;
Boutin, RD ;
Burstein, D .
RADIOLOGY, 1997, 205 (02) :551-558
[7]
Correlation of radiographic and MRI parameters to morphological and biochemical assessment of intervertebral disc degeneration [J].
Benneker, LM ;
Heini, PF ;
Anderson, SE ;
Alini, M ;
Ito, K .
EUROPEAN SPINE JOURNAL, 2005, 14 (01) :27-35
[8]
The pathophysiology of the intervertebral disc [J].
Bibby, SRS ;
Jones, DA ;
Lee, RB ;
Yu, J ;
Urban, JPG .
JOINT BONE SPINE, 2001, 68 (06) :537-542
[9]
Nucleus pulposus glycosaminoglycan content is correlated with axial mechanics in rat lumbar motion segments [J].
Boxberger, John I. ;
Sen, Sounok ;
Yerramalli, Chandra S. ;
Elliott, Dawn M. .
JOURNAL OF ORTHOPAEDIC RESEARCH, 2006, 24 (09) :1906-1915
[10]
SPINE UPDATE - AGING AND DEGENERATION OF THE HUMAN INTERVERTEBRAL DISC [J].
BUCKWALTER, JA .
SPINE, 1995, 20 (11) :1307-1314