Reciprocal Repression between Sox3 and Snail Transcription Factors Defines Embryonic Territories at Gastrulation

被引：77

作者：

Acloque, Herve ^{[1
]}

Ocana, Oscar H. ^{[1
]}

Matheu, Ander ^{[2
]}

Rizzoti, Karine ^{[2
]}

Wise, Clare ^{[2
]}

Lovell-Badge, Robin ^{[2
]}

Angela Nieto, M. ^{[1
]}

机构：

[1] CSIC UMH, Inst Neurociencias, Alacant 03550, Spain

[2] Natl Inst Med Res, London NW7 1AA, England

来源：

DEVELOPMENTAL CELL | 2011年 / 21卷 / 03期

基金：

英国医学研究理事会;

关键词：

EPITHELIAL-MESENCHYMAL TRANSITIONS; SITU HYBRIDIZATION ANALYSIS; PRIMITIVE STREAK; CHICK-EMBRYOS; WHOLE-MOUNT; GENES; EXPRESSION; CELLS; DIFFERENTIATION; MESODERM;

D O I：

10.1016/j.devcel.2011.07.005

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

In developing amniote embryos, the first epithelial-to-mesenchymal transition (EMT) occurs at gastrulation, when a subset of epiblast cells moves to the primitive streak and undergoes EMT to internalize and generate the mesoderm and the endoderm. We show that in the chick embryo this decision to internalize is mediated by reciprocal transcriptional repression of Snail2 and Sox3 factors. We also show that the relationship between Sox3 and Snail is conserved in the mouse embryo and in human cancer cells. In the embryo, Snail-expressing cells ingress at the primitive streak, whereas Sox3-positive cells, which are unable to ingress, ensure the formation of ectodermal derivatives. Thus, the subdivision of the early embryo into the two main territories, ectodermal and mesendodermal, is regulated by changes in cell behavior mediated by the antagonistic relationship between Sox3 and Snail transcription factors.

引用

页码：546 / 558

页数：13

共 56 条

[1] In situ hybridization analysis of chick embryos in whole-mount and tissue sections [J].