Relationship among oxidative DNA damage, gastric mucosal density and the relevance of cagA, vacA and iceA genotypes of Helicobacter pylori

被引：19

作者：

Ladeira, Marcelo S. P. ^{[2
]}

Bueno, Roberta C. A. ^{[3
]}

Dos Santos, Bruna Fornazari ^{[3
]}

Pinto, Carla L. S. ^{[2
,3
]}

Prado, Renato P. ^{[2
,3
]}

Silveira, Marcela G. ^{[2
,3
]}

Rodrigues, Maria A. M. ^{[4
]}

Bartchewsky, Waldemar, Jr. ^{[1
]}

Pedrazzoli, Jose, Jr. ^{[1
]}

Ribeiro, Marcelo Lima ^{[1
]}

Salvadori, Daisy M. F. ^{[3
]}

机构：

[1] Univ Sao Francisco, Unid Integrad Farmacol & Gastroenterol, Braganca Paulista, SP, Brazil

[2] UNESP, Dept Clin Med, Lab Biol Mol Clin Med, Botucatu, SP, Brazil

[3] UNESP, Dept Patol, Lab Toxigenom & Epidemiol Mol, Botucatu, SP, Brazil

[4] UNESP, Fac Med, Dept Patol, Botucatu, SP, Brazil

来源：

DIGESTIVE DISEASES AND SCIENCES | 2008年 / 53卷 / 01期

基金：

巴西圣保罗研究基金会;

关键词：

oxidative DNA damage; Helicobacter pylori; real-time PCR; Comet assay; gastric cancer; inflammation;

D O I：

10.1007/s10620-007-9850-0

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

The aim of this study was to evaluate the relationship among oxidative DNA damage, density of Helicobacter pylori and the relevance of cagA, vacA and iceA genotypes of H. pylori. Gastric epithelial cells were isolated from 24 uninfected patients, 42 H. pylori infected patients with gastritis, and 61 patients with gastric cancer. Oxidative DNA damage was analyzed by the Comet assay, the density of H. pylori was measured by real-time polymerase chain reaction (PCR), and allelic variants of cagA, vacA and iceA were identified using the PCR. Infected patients by Helicobacter pylori cagA(+), vacAs1 m1 and iceA1 genotype showed higher levels of oxidative DNA damage than infected patients with H. pylori cagA(-), vacAs2 m2 and iceA2 genotypes and uninfected patients. Density of H. pylori did not influence oxidative DNA damage. Our results indicate that H. pylori genotype is more relevant than density for oxidative DNA damage.

引用

页码：248 / 255

页数：8

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