Probiotics Prevent the Development of 1,2-Dimethylhydrazine (DMH)-Induced Colonic Tumorigenesis through Suppressed Colonic Mucosa Cellular Proliferation and Increased Stimulation of Macrophages

被引:52
作者
Foo, Ning-Ping [1 ,4 ,5 ,6 ]
Yang, Hui Ou [1 ]
Chiu, Hsueh-Huei [2 ]
Chan, Hing-Yuen [2 ]
Liao, Chii-Cherng [2 ]
Yu, Chung-Keung [3 ,7 ,8 ]
Wang, Ying-Jan [1 ]
机构
[1] Natl Cheng Kung Univ, Coll Med, Dept Environm & Occupat Hlth, Tainan 70428, Taiwan
[2] Food Ind Res & Dev Inst, Bioresource Collect & Res Ctr BCRC, Hsinchu, Taiwan
[3] Natl Cheng Kung Univ, Coll Med, Dept Microbiol & Immunol, Tainan 70428, Taiwan
[4] Chi Mei Med Ctr, Dept Emergency Med, Tainan, Taiwan
[5] Chung Hwa Univ Med Technol, Dept Early Childhood Caring & Educ, Tainan, Taiwan
[6] Ditmanson Med Fdn Chiayi Christian Hosp, Dept Emergency Med, Chiayi, Taiwan
[7] Natl Cheng Kung Univ, Coll Med, Infect Dis & Signaling Res Ctr, Tainan 70428, Taiwan
[8] Natl Appl Res Labs, Natl Lab Anim Ctr, Taipei, Taiwan
关键词
probiotics; DMH; colon tumor; ACF; immunity; ABERRANT CRYPT FOCI; BIFIDOBACTERIUM-LONGUM; INTERMEDIATE BIOMARKERS; LACTOBACILLUS-CASEI; INTESTINAL TUMORS; S-PHASE; CANCER; MICE; ENHANCEMENT; PHAGOCYTOSIS;
D O I
10.1021/jf203444d
中图分类号
S [农业科学];
学科分类号
082806 [农业信息与电气工程];
摘要
Probiotics modulate immunity and inhibit colon carcinogenesis in experimental models, but these effects largely depend on the bacterial strain, and the precise mechanisms are not well understood. Therefore, we studied the effect of Bifidobacterium longum and/or Lactobacillus gasseri on the development of 1,2-dimethylhydrazine (DMH)-induced colonic precancerous lesions and tumors in mice while delineating the possible mechanisms involved. The results suggest that dietary consumption of probiotics (B. longum and L. gasser resulted in a significant inhibition of DMH-induced aberrant crypt foci (ACF) formation in male ICR mice. Long-term (24 weeks) dietary consumption of probiotics resulted in a reduction of colon tumor multiplicity and the size of the tumors. Administration of B. longum and L. gasseri suppressed the rate of colonic mucosa cellular proliferation in a manner correlating with the inhibition of tumor induction by DMH. In addition, the phagocytic activity of peritoneal macrophages was significantly increased in the DMH-treated mice that were fed various doses of B. longum, but not with L. gasseri or combined probiotics (B. longum + L. gasseri). We also found that L. gasseri significantly increased the proliferation of RAW264.7 macrophage cells through an increase in S phase DNA synthesis, which was related to the up-regulation of proliferating cell nuclear antigen (PCNA) and cyclin A. Taken together, these results demonstrate the in vivo chemopreventive efficacy and the immune stimulating mechanisms of dietary probiotics against DMH-induced colonic tumorigenesis.
引用
收藏
页码:13337 / 13345
页数:9
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