Single-cell analysis of λ immunity regulation

被引：40

作者：

Bæk, K

Svenningsen, S

Eisen, H

Sneppen, K

Brown, S

机构：

[1] Univ Copenhagen, Dept Mol Cell Biol, DK-1353 Copenhagen K, Denmark

[2] Fred Hutchinson Canc Res Ctr, Seattle, WA 98109 USA

[3] Niels Bohr Inst, DK-2100 Copenhagen O, Denmark

来源：

JOURNAL OF MOLECULAR BIOLOGY | 2003年 / 334卷 / 03期

关键词：

bacteriophage lambda; repressor; GFP; single-cell analyses;

D O I：

10.1016/j.jmb.2003.09.037

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have examined expression of the lambdacI operon in single cells via a rex:: gfp substitution. Although average fluorescence agreed with expectations for expression of X-repressor, fluorescence fluctuated greatly from cell-to-cell. Fluctuations in repressor concentration are not predicted by previous models and are tolerated in part by a regulatory response to DNA damage. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：363 / 372

页数：10

共 37 条

[1]

Aurell E, 2002, PHYS REV E, V65, DOI 10.1103/PhysRevE.65.051914

[2] Crystal structure of the λ repressor C-terminal domain provides a model for cooperative operator binding [J].